| Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle. | |
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MedLine Citation:
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PMID: 21653224 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Phospholemman (PLM, FXYD1) is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). We explored the impact of acute and short-term training exercise on PLM physiology in human skeletal muscle. A group of moderately trained males (n=8) performed a one-hour acute bout of exercise by utilizing a one-legged cycling protocol. Muscle biopsies were taken from vastus lateralis at 0 and 63 min (non-exercised leg) and 30 and 60 min (exercised leg). In a group of sedentary males (n=9), we determined the effect of a 10-day intense aerobic cycle training on Na+,K+-ATPase subunit expression, PLM phosphorylation and total PLM expression as well as PLM phosphorylation in response to acute exercise (1 h at ~72% VO2peak). Biopsies were taken at rest, immediately following, and 3 h after an acute exercise bout before and at the conclusion of the 10-day training study. PLM phosphorylation was increased both at Ser63 and Ser68 immediately after acute exercise (75%; p<0.05 and 30%; p<0.05, respectively). Short-term training had no adaptive effect on PLM phosphorylation at Ser63 and Ser68 nor was the total amount of PLM altered post-training. The protein expression of α1, α2 and β1-subunits of Na+,K+-ATPase were increased after training (113%; p<0.05, 49%; p<0.05 and 27%; p<0.05 respectively). While an acute bout of exercise increased the phosphorylation of PKCα/βII on Thr638/641 pre- and post-training, phosphorylation of PKC-ζ/λ on Thr403/410 was increased in response to acute exercise only after the 10-day training. In conclusion, we show that only acute exercise, and not short-term training, increases phosphorylation of PLM on Ser63 and Ser68 and data from one-legged cycling indicate that this effect of exercise on PLM phosphorylation is not due to systemic factors. Our results provide evidence that phosphorylation of PLM may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. |
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Authors:
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Boubacar Benziane; Ulrika Widegren; Sergej Pirkmajer; Jan Henriksson; Nigel K Stepto; Alexander V Chibalin |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-7 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: - ISSN: 1522-1555 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Karolinska Insitutet. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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