| Effect of exercise training on nitric oxide and superoxide/H₂O₂ signaling pathways in collateral-dependent porcine coronary arterioles. | |
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MedLine Citation:
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PMID: 22323648 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endothelial nitric oxide (NO) synthase (NOS) has been shown to contribute to enhanced vascular function after exercise training. Recent studies have revealed that relatively low concentrations of reactive oxygen species can contribute to endothelium-dependent vasodilation under physiological conditions. We tested the hypothesis that exercise training enhances endothelial function via endothelium-derived vasodilators, NO and superoxide/H(2)O(2), in the underlying setting of chronic coronary artery occlusion. An ameroid constrictor was placed around the proximal left circumflex coronary artery to induce gradual occlusion in Yucatan miniature swine. At 8 wk postoperatively, pigs were randomly assigned to sedentary (pen-confined) or exercise-training (treadmill-run: 5 days/wk for 14 wk) regimens. Exercise training significantly enhanced concentration-dependent, bradykinin-mediated dilation in cannulated collateral-dependent arterioles (∼130 μm diameter) compared with sedentary pigs. NOS inhibition reversed training-enhanced dilation at low bradykinin concentrations in collateral-dependent arterioles, although increased dilation persisted at higher bradykinin concentrations. Total and phosphorylated (Ser(1179)) endothelial NOS protein levels were significantly increased in arterioles from collateral-dependent compared with the nonoccluded region, independent of exercise. The H(2)O(2) scavenger polyethylene glycol-catalase abolished the training-enhanced bradykinin-mediated dilation in collateral-dependent arterioles; similar results were observed with the SOD inhibitor diethyldithiocarbamate. Fluorescence measures of bradykinin-stimulated H(2)O(2) levels were significantly increased by exercise training, independent of occlusion. The NADPH inhibitor apocynin significantly attenuated bradykinin-mediated dilation in arterioles of exercise-trained, but not sedentary, pigs and was associated with significantly increased protein levels of the NADPH subunit p67phox. These data provide evidence that, in addition to NO, the superoxide/H(2)O(2) signaling pathway significantly contributes to exercise training-enhanced endothelium-mediated dilation in collateral-dependent coronary arterioles. |
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Authors:
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Wei Xie; Janet L Parker; Cristine L Heaps |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-02-09 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 112 ISSN: 1522-1601 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-05-02 Completed Date: 2012-08-27 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1546-55 Citation Subset: IM |
Affiliation:
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Department of Veterinary Physiology and Pharmacology, Texas A & M University, College Station, TX 77843, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arterioles / metabolism, physiopathology Collateral Circulation* / drug effects Coronary Circulation* / drug effects Coronary Occlusion / metabolism*, physiopathology Coronary Vessels / drug effects, metabolism*, physiopathology Disease Models, Animal Dose-Response Relationship, Drug Endothelium, Vascular / metabolism, physiopathology Enzyme Inhibitors / pharmacology Female Free Radical Scavengers / pharmacology Hydrogen Peroxide / metabolism* Microscopy, Fluorescence Microscopy, Video Muscle, Smooth, Vascular / metabolism, physiopathology NADPH Oxidase / antagonists & inhibitors, metabolism Nitric Oxide / metabolism* Nitric Oxide Synthase Type III / antagonists & inhibitors, metabolism Phosphorylation Physical Exertion* Signal Transduction* / drug effects Superoxides / metabolism* Swine Swine, Miniature Time Factors Vasodilation Vasodilator Agents / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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R01-GM-046441/GM/NIGMS NIH HHS; R01-HL-064931/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Free Radical Scavengers; 0/Vasodilator Agents; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides; 7722-84-1/Hydrogen Peroxide; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.6.3.1/NADPH Oxidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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