Document Detail


Effect of exercise on glutamine synthesis and transport in skeletal muscle from rats.
MedLine Citation:
PMID:  19207717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Reductions in plasma glutamine are observed after prolonged exercise. Three hypotheses can explain such a decrease: (i) high demand by the liver and kidney; (ii) impaired release from muscles; and (iii) decreased synthesis in skeletal muscle. The present study investigated the effects of exercise on glutamine synthesis and transport in rat skeletal muscle. 2. Rats were divided into three groups: (i) sedentary (SED; n = 12); (ii) rats killed 1 h after the last exercise bout (EX-1; n = 15); and (iii) rats killed 24 h after the last exercise bout (EX-24; n = 15). Rats in the trained groups swam 1 h/day, 5 days/week for 6 weeks with a load equivalent to 5.5% of their bodyweight. 3. Plasma glutamine and insulin were lower and corticosterone was higher in EX-1 compared with SED rats (P < 0.05 and P < 0.01, respectively). Twenty-four hours after exercise (EX-24), plasma glutamine was restored to levels seen in SED rats, whereas insulin levels were higher (P < 0.001) and corticosterone levels were lower (P < 0.01) than in EX-1. In the soleus, ammonia levels were lower in EX-1 than in SED rats (P < 0.001). After 24 h, glutamine, glutamate and ammonia levels were lower in EX-24 than in SED and EX-1 rats (P < 0.001). Soleus glutamine synthetase (GS) activity was increased in EX-1 and was decreased in EX-24 compared with SED rats (both P < 0.001). 4. The decrease in plasma glutamine concentration in EX-1 is not mediated by GS or glutamine transport in skeletal muscle. However, 24 h after exercise, lower GS may contribute to the decrease in glutamine concentration in muscle.
Authors:
Ronaldo V T dos Santos; Erico C Caperuto; Marco T de Mello; Miguel L Batista; Luis F B P C Rosa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-17
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  36     ISSN:  1440-1681     ISO Abbreviation:  Clin. Exp. Pharmacol. Physiol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-14     Completed Date:  2010-02-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  770-5     Citation Subset:  IM    
Affiliation:
Department of Bioscience, Federal University of S??o Paulo, Baixada Santista, Santos, Brazil. ronaldo.thomatieli@unifesp.br
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MeSH Terms
Descriptor/Qualifier:
Ammonia / metabolism
Animals
Corticosterone / blood
Glutamate-Ammonia Ligase / metabolism
Glutamic Acid / metabolism
Glutamine / biosynthesis*,  blood,  metabolism
Insulin / blood
Male
Muscle, Skeletal / metabolism*
Physical Conditioning, Animal*
Protein Transport
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
11061-68-0/Insulin; 50-22-6/Corticosterone; 56-85-9/Glutamine; 56-86-0/Glutamic Acid; 7664-41-7/Ammonia; EC 6.3.1.2/Glutamate-Ammonia Ligase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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