| Effect of ethyl alcohol on growth and intracellular alanine racemase of psychrotrophs. | |
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MedLine Citation:
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PMID: 16232459 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The psychrotrophic alanine racemase from Pseudomonas fluorescens, a typical psychrotroph, is less resistant to organic solvents than the enzymes from thermophilic and mesophilic bacteria (Okubo et al., J. Home Econ. Jpn., 46: 1135-1140, 1995). To further elucidate this difference, we examined the effect of ethyl alcohol on the growth and intracellular alanine racemase activity of three typical psychrotrophs-P. fluorescens, Bacillus psychrosaccharolyticus and B. psychrophilus-in comparison with two mesophiles, Escherichia coli and B. subtilis. Although all the bacteria grew to the early stationary phase when cultivated at 22 degrees C for 36 h in the absence of ethyl alcohol, the growth of the psychrotrophs was more effectively suppressed by the addition of 3 and 5% ethyl alcohol to the medium than that of the mesophiles. The intracellular alanine racemase activity of the psychrotrophs was also more markedly reduced in the presence of ethyl alcohol than that of the mesophiles. When bacterial cells of each strain grown at 22 degrees C for 36 h in the absence of alcohol were suspended in 0-5 % ethyl alcohol solution and incubated at 30 degrees C for 1 h, both the survival ratio and intracellular alanine racemase activity of the psychrotrophs were lower than those of the mesophiles. Thus, ethyl alcohol effectively reduced both the growth of the psychrotrophs and their intracellular alanine racemase activity. Low concentrations of various other alcohols also repressed the growth of the psychrotrophs at 10 degrees C. |
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Authors:
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Y Okubo; K Yokoigawa; H Kawai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: 87 ISSN: 1389-1723 ISO Abbreviation: J. Biosci. Bioeng. Publication Date: 1999 |
Date Detail:
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Created Date: 2005-10-19 Completed Date: 2005-10-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: Japan |
Other Details:
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Languages: eng Pagination: 241-4 Citation Subset: - |
Affiliation:
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Department of Food Science and Nutrition, Nara Women's University, Nara 630-8506, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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