| Effect of ethanol on lecithin:cholesterol acyltransferase (LCAT) activity. | |
| | |
MedLine Citation:
|
PMID: 3992006 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effect of variable doses of ethanol on plasma lecithin: cholesterol acyltransferase (LCAT) activity was examined in male, atherosclerosis-susceptible squirrel monkeys over a 12-month period. Primates were divided into three groups: 1) Controls fed isocaloric liquid diet; 2) Low Ethanol monkeys given liquid diet with vodka substituted isocalorically for carbohydrate at 12% of calories; and 3) High Ethanol animals fed diet plus vodka at 24% of calories. There were no significant differences between the treatments in serum glutamate oxaloacetate transaminase (SGOT), a measure of liver function. However, plasma LCAT activity (% esterification/min) measured in vitro was significantly reduced in High Ethanol monkeys while cholesterol esterification was elevated in the Low Ethanol group and intermediate in Controls. Similarly, the in vivo appearance of radiolabeled cholesteryl ester in high density lipoproteins (HDL) following the intravenous injection of 3H mevalonolactone was highest in the Low Ethanol primates, intermediate in Controls and significantly lower in monkeys fed the high alcohol diet. In vitro measurement of LCAT enzyme efficiency was similar for the three groups while substrate efficiency was lower in the High Ethanol treatment. Although LCAT activator (apoprotein A-I) was not markedly altered by dietary ethanol and the concentration of LCAT substrates (HDL free cholesterol and phosphatidyl choline) was significantly elevated in the High Ethanol group, subtle modifications in substrate-product composition may account for the observed reduction in cholesterol esterification. These include potential substrate and/or product LCAT inhibition resulting from increased concentrations of plasma free cholesterol, HDL lysophosphatidyl choline, and higher HDL2/HDL3 subfraction ratios, as well as alterations in HDL phospholipid fatty acid profiles in the High Ethanol group. Results from this study provide the first evidence of an anomalous enhancement in LCAT activity in nonhuman primates fed ethanol at 12% of calories and a marked depression in cholesterol esterification at the 24% dose which may be due to substrate alterations and product inhibition prior to overt biochemical evidence of liver dysfunction. |
| | |
Authors:
|
J J Mulligan; J E Cluette-Brown; R Noring; F D Igoe; J Chong; J L Hojnacki |
Related Documents
:
|
15499896 - Nutrient intakes, nutritional patterns and the risk of liver cirrhosis: an explorative ... 10456566 - The effect of fat and carbohydrate content of the diet on voluntary ethanol intake in g... 3263456 - Flow cytometric and histological analysis of mouse thymus in fetal alcohol syndrome. 6634876 - Long-lasting reduction in ethanol selection after involuntary intake of ethanol/chlordi... 8745736 - Effects of iron deficiency on the hepatic development of plasmodium yoelii. 8674486 - Anthropometric measurements of the nilotic tribes in a refugee camp. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Research communications in chemical pathology and pharmacology Volume: 47 ISSN: 0034-5164 ISO Abbreviation: Res. Commun. Chem. Pathol. Pharmacol. Publication Date: 1985 Feb |
Date Detail:
|
Created Date: 1985-06-10 Completed Date: 1985-06-10 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0244734 Medline TA: Res Commun Chem Pathol Pharmacol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 181-202 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cholesterol Esters / analysis Cholesterol, HDL / analysis Ethanol / pharmacology* Lipoproteins, HDL / analysis Lysophosphatidylcholines / metabolism Male Phosphatidylcholine-Sterol O-Acyltransferase / analysis* Phospholipids / analysis Saimiri |
| Grant Support | |
ID/Acronym/Agency:
|
RR-00168/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cholesterol Esters; 0/Cholesterol, HDL; 0/Lipoproteins, HDL; 0/Lysophosphatidylcholines; 0/Phospholipids; 64-17-5/Ethanol; EC 2.3.1.43/Phosphatidylcholine-Sterol O-Acyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of ethanol feeding on hepatic collagen synthesis and degradation in rats.
Next Document: Ethanol induced changes in lipid peroxidation and nonprotein sulfhydryl content. Different sensitivi...