Document Detail


Effect of ethanol exposure on circulating levels of insulin-like growth factor I and II, and insulin-like growth factor binding proteins in fetal rats.
MedLine Citation:
PMID:  7509575     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Maternal ethanol (ETOH) exposure is associated with impaired fetal growth. Because insulin-like growth factors (IGFs) are thought to be important in the regulation of fetal somatic growth, we examined the influence of maternal ETOH exposure on fetal growth and plasma levels of IGF-I, IGF-II, and IGF binding proteins (IGFBPs) in the rat model. Control (A) dams were fed a standard rat chow ad libitum. ETOH (E) consuming dams were fed a 36% ETOH diet, and pair-fed (P) dams were fed isocaloric amounts of a control liquid diet. All animals were killed on day 20 of gestation. Plasma concentrations of IGF-I and -II were determined by radioimmunoassay after formic acid-acetone extraction and heat inactivation of IGFBPs. Levels of IGFBPs in fetal plasma were estimated by Western ligand blotting after protein separation by SDS-PAGE and electrotransfer to nitrocellulose. Membranes were probed with [125I]IGF-I, and IGFBPs were identified by autoradiography, quantified by scanning densitometry and results expressed relative to corresponding IGFBPs in control fetal plasma. Maternal weight gain from conception to 20 days of pregnancy was reduced for E compared to P and A dams (p < 0.05 E vs. P or A). The same pattern was reflected in fetal weight that tended to be lower in P compared with A pups, and was significantly reduced in E pups compared with both groups (p < 0.0001 E vs. P or A). Thus, fetal growth was more retarded in E animals despite equal caloric and protein intake by E and P dams.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
H J Mauceri; T Unterman; S Dempsey; W H Lee; S Conway
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Alcoholism, clinical and experimental research     Volume:  17     ISSN:  0145-6008     ISO Abbreviation:  Alcohol. Clin. Exp. Res.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-03-31     Completed Date:  1994-03-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7707242     Medline TA:  Alcohol Clin Exp Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1201-6     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Northern Illinois University, DeKalb 60115.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Weight / drug effects,  physiology
Carrier Proteins / blood*
Ethanol / toxicity
Female
Fetal Alcohol Syndrome / blood*
Fetal Growth Retardation / blood
Insulin-Like Growth Factor Binding Proteins
Insulin-Like Growth Factor I / physiology*
Insulin-Like Growth Factor II / physiology*
Male
Pregnancy
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
R03 AA08000 01A1S1/AA/NIAAA NIH HHS; R03 AA08000-01A1/AA/NIAAA NIH HHS; R15 HD25684-01/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Insulin-Like Growth Factor Binding Proteins; 64-17-5/Ethanol; 67763-96-6/Insulin-Like Growth Factor I; 67763-97-7/Insulin-Like Growth Factor II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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