Document Detail

Effect of essential amino acids on mouse embryo viability and ammonium production.
MedLine Citation:
PMID:  11665668     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To examine the effect of essential amino acids concentrations on mouse embryo development.
METHODS: Mouse embryos were cultured in medium with different concentrations of essential amino acids and development to the blastocyst stage and viability assessed. Ammonium production resulting from medium breakdown and amino acid metabolism by embryos were also assessed.
RESULTS: Reducing the essential amino acid concentration significantly increased blastocyst development and cell numbers. Lowering the essential amino acid concentration decreased ammonium production in the medium.
CONCLUSIONS: Culture media for the development of preimplantation embryos should have a reduced essential amino acid concentration to facilitate embryo development.
M Lane; K Hooper; D K Gardner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  18     ISSN:  1058-0468     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-10-22     Completed Date:  2002-03-01     Revised Date:  2013-03-26    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  519-25     Citation Subset:  IM    
Colorado Center for Reproductive Medicine, Research and Development, Englewood, Colorado, USA.
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MeSH Terms
Amino Acids, Essential / pharmacology*
Cells, Cultured
Culture Media / chemistry
Embryo Transfer
Embryo, Mammalian / cytology*,  drug effects,  physiology*
Embryonic and Fetal Development
Fetal Death
Mice, Inbred Strains
Quaternary Ammonium Compounds / metabolism*
Reg. No./Substance:
0/Amino Acids, Essential; 0/Culture Media; 0/Quaternary Ammonium Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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