Document Detail

Effect of esophageal ligation on small intestinal development in normal and growth-retarded fetal rabbits.
MedLine Citation:
PMID:  16954949     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: The uninterrupted passage of amniotic fluid through the gastrointestinal tract is hypothesized to influence both intestinal and overall fetal somatic development. The effect of in utero esophageal ligation (EL) and therefore the exclusion of AF on somatic growth, small intestinal (SI) morphology and proliferation, and the expression of the glucose transporter sodium-glucose cotransporter 1 (SGLT-1) in both normal and intrauterine growth-retarded (IUGR) fetal rabbits were evaluated. METHODS: Thirteen pregnant New Zealand white rabbits underwent surgery on day 24 of their normal 31-day gestation. Ipsilateral normal and IUGR fetuses underwent EL; the contralateral normal and IUGR fetuses underwent cervical exploration only forming 4 study groups (control-normal, control-IUGR, EL-normal and EL-IUGR). Rabbits were killed on day 31. Small intestinal villus height was measured, and epithelial cell proliferation was deter mined by proliferating cell nuclear antigen staining. Sodium-glucose cotransporter 1 messenger RNA (mRNA) and protein expressions were analyzed. Statistical analysis was performed using 2-way analysis of variance. RESULTS: Esophageal ligation reduced fetal weight in IUGR by 15% and in normal by 10%. Villus height was significantly reduced in IUGR versus normal in both control and EL (control, P = 0.01; EL, P = 0.05). Intrauterine growth-retarded fetuses had reduced SI proliferation versus normal in both control and EL. Sodium-glucose cotransporter 1 mRNA production in EL fetuses was equal to control fetuses. Esophageal ligation-normal and EL-IUGR fetuses exhibited reduced protein levels and decreased staining for SGLT-1 in villus enterocytes. CONCLUSIONS: Amniotic fluid exclusion by in utero EL reduced fetal weight. Small intestinal proliferation was not affected by EL. Although SGLT-1 mRNA and protein were produced in all 4 groups, exposure of the fetal gastrointestinal tract to amniotic fluid appears necessary for proper brush border expression of nutrient transporter proteins.
Christina Cellini; Jian Xu; Terry L Buchmiller
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  43     ISSN:  1536-4801     ISO Abbreviation:  J. Pediatr. Gastroenterol. Nutr.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-06     Completed Date:  2006-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  291-8     Citation Subset:  IM    
Division of Pediatric Surgery, Children's Hospital of New York Presbyterian-Weill Medical College of Cornell University, New York, NY, USA.
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MeSH Terms
Amniotic Fluid / physiology
Blotting, Western
Cell Division
Disease Models, Animal
Enterocytes / pathology
Epithelial Cells / pathology
Esophageal Atresia / physiopathology
Esophagus / embryology*,  surgery*
Fetal Growth Retardation / physiopathology*
Fetal Weight
Gene Expression
Gestational Age
Intestine, Small / embryology*
RNA, Messenger / analysis
Sodium-Glucose Transporter 1 / analysis,  genetics
Reg. No./Substance:
0/RNA, Messenger; 0/Sodium-Glucose Transporter 1

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