Document Detail


Effect of epinephrine on glucose metabolism and hydrogen peroxide content in incubated rat macrophages.
MedLine Citation:
PMID:  1472089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of epinephrine on glucose metabolism and hydrogen peroxide content were examined in incubated rat macrophages. An increase in the activities of hexokinase and citrate synthase and a reduction in that of glucose-6-phosphate dehydrogenase was found in resident, inflammatory and activated macrophages incubated for 1 hr in the presence of epinephrine. Glucose utilization by incubated resident, inflammatory and activated macrophages was augmented markedly by the addition of epinephrine, whereas lactate formation was depressed. Under the same conditions, there was a 2.6-fold increment of hydrogen peroxide content and of [U-14C]glucose decarboxylation in activated macrophages incubated for 40 min. Similar results were obtained when pyruvate and oxoglutarate was substituted for glucose. These findings suggest that epinephrine may increase hydrogen peroxide production in activated macrophages possibly through a mitochondrial mechanism other than the pentose-phosphate pathway. Between 40 and 90 min of incubation, the content of hydrogen peroxide decreased markedly, and there was no detectable glucose utilization in the presence of epinephrine. These observations are consistent with the idea that this catecholamine stimulates both hydrogen peroxide production and metabolism, the first process being dependent on mitochondrial fuels.
Authors:
L F Costa Rosa; D A Safi; Y Cury; R Curi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  44     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-01-28     Completed Date:  1993-01-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2235-41     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, University of São Paulo, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Radioisotopes / diagnostic use
Cells, Cultured
Citrate (si)-Synthase / metabolism
Epinephrine / pharmacology*
Glucose / metabolism*
Glucosephosphate Dehydrogenase / metabolism
Hexokinase / metabolism
Hydrogen Peroxide / metabolism*
Ketoglutaric Acids / metabolism
Kinetics
Lactates / metabolism
Lactic Acid
Macrophage Activation / physiology
Macrophages / drug effects*,  enzymology,  metabolism*
Male
Peritoneal Cavity / cytology
Pyruvates / metabolism
Pyruvic Acid
Rats
Rats, Wistar
Stimulation, Chemical
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Ketoglutaric Acids; 0/Lactates; 0/Pyruvates; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 50-99-7/Glucose; 51-43-4/Epinephrine; 7722-84-1/Hydrogen Peroxide; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 2.3.3.1/Citrate (si)-Synthase; EC 2.7.1.1/Hexokinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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