Document Detail

Effect of environmental pH on the efficiency of cellular hybridization.
MedLine Citation:
PMID:  4340167     Owner:  NLM     Status:  MEDLINE    
The hybridization of a human and mouse cell was strikingly pH-dependent, with a well defined optimum at (about) pH 7.6-8.0. The yield of hybrid cell colonies (1 per 500-2000 heterokaryocytes) was several hundred times greater than that obtained at pH 6.8-7.2. Although there was a significant effect on the efficiency of cell fusion, the critical time for the pH effect was in the first 4-8 days after fusion, presumably while viable hybrids were being formed from the multinucleated heterokaryocytes.
C M Croce; H Koprowski; H Eagle
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Jul 
Date Detail:
Created Date:  1972-09-22     Completed Date:  1972-09-22     Revised Date:  2013-06-12    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1953-6     Citation Subset:  IM    
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MeSH Terms
Cell Fusion
Cell Line
Cell Transformation, Neoplastic
Cells, Cultured / drug effects
Hybrid Cells*
Hybridization, Genetic
Hydrogen-Ion Concentration
L Cells (Cell Line) / drug effects
Lysophosphatidylcholines / pharmacology
Parainfluenza Virus 1, Human
Time Factors
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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