Document Detail


Effect of the entero-pancreatic hormones, gastric inhibitory polypeptide and glucagon-like polypeptide-1(7-36) amide, on fatty acid synthesis in explants of rat adipose tissue.
MedLine Citation:
PMID:  1919397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of gastric inhibitory polypeptide (GIP), glucagon-like peptide-1(7-36) amide, (GLP-1(7-36) amide), glucagon-like peptide-2 (GLP-2), glucagon and insulin on fatty acid synthesis in explants of rat adipose tissue from various sites was investigated. GIP, GLP-1(7-36) amide and insulin stimulated fatty acid synthesis, as determined by measuring the incorporation of [14C]acetate into saponifiable fat, in a dose-dependent manner, over the concentration range 5-15 ng/ml (0.87-2.61 nmol/l) for insulin and 0.5-7.5 ng/ml for GIP (0.10-1.50 nmol/l) and GLP-1(7-36) amide (0.15-2.27 nmol/l). Insulin and GIP caused a significantly greater stimulation of [14C]acetate incorporation into fatty acids in omental adipose tissue than in either epididymal or subcutaneous adipose tissue. Both GIP and GLP-1(7-36) amide had the ability to stimulate fatty acid synthesis within the physiological range of the circulating hormones. At lower concentrations of the hormones, GLP-1(7-36) amide was a more potent stimulator of fatty acid synthesis than GIP in omental adipose tissue culture; the basal rate of fatty acid synthesis was 0.41 +/- 0.03 pmol acetate incorporated/mg wet weight tissue per 2 h; at 0.10 nmol hormone/1 1.15 +/- 0.10 and 3.40 +/- 0.12 pmol acetate incorporated/mg wet weight tissue per 2 h for GIP and GLP-1(7-36) amide respectively (P less than 0.01). GLP-2 and glucagon were without effect on fatty acid synthesis in omental adipose tissue. The study indicates that GIP and GLP-1(7-36)amide, in addition to stimulating insulin secretion, may play a direct physiological role in vivo, in common with insulin, in promoting fatty acid synthesis in adipose tissue.
Authors:
J Oben; L Morgan; J Fletcher; V Marks
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  130     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1991-11-01     Completed Date:  1991-11-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  267-72     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, University of Surrey, Guildford.
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / drug effects,  metabolism*
Animals
Culture Techniques
Fatty Acids / biosynthesis*
Gastric Inhibitory Polypeptide / pharmacology
Glucagon / pharmacology
Glucagon-Like Peptide 1
Glucagon-Like Peptides
Insulin / pharmacology
Male
Neuropeptides / pharmacology*
Peptide Fragments / pharmacology
Peptides / pharmacology
Rats
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Neuropeptides; 0/Peptide Fragments; 0/Peptides; 11061-68-0/Insulin; 119637-73-9/glucagon-like peptide 1 (7-36)amide; 59392-49-3/Gastric Inhibitory Polypeptide; 62340-29-8/Glucagon-Like Peptides; 89750-14-1/Glucagon-Like Peptide 1; 9007-92-5/Glucagon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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