Document Detail


Effect of enhanced red blood cell aggregation on blood flow resistance in an isolated-perfused guinea pig heart preparation.
MedLine Citation:
PMID:  16369087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of red blood cell (RBC) aggregation as a determinant of in vivo blood flow is still unclear. This study was designed to investigate the influence of a well-controlled enhancement of RBC aggregation on blood flow resistance in an isolated-perfused heart preparation. Guinea pig hearts were perfused through a catheter inserted into the root of the aorta using a pressure servo-controlled pump system that maintained perfusion pressures of 30 to 100 mmHg. The hearts were beating at their intrinsic rates and pumping against the perfusion pressure. RBC aggregation was increased by Pluronic (F98) coating of RBC at a concentration 0.025 mg/ml, corresponding to about a 100% increment in RBC aggregation as measured by erythrocyte sedimentation rate. Isolated heart preparations were perfused with 0.40 l/l hematocrit unmodified guinea pig blood and with Pluronic-coated RBC suspensions in autologous plasma. At high perfusion pressures there were no significant differences between the flow resistance values for the two perfusates, with differences in flow resistance only becoming significant at lower perfusion pressures. These results can be interpreted to reflect the shear dependence of RBC aggregation: higher shear forces associated with higher perfusion pressures should have dispersed RBC aggregates resulting in blood flow resistances similar to control values. Experiments repeated in preparations in which the smooth muscle tone was inhibited by pre-treatment with papaverine indicated that significant effects of enhanced RBC aggregation could be detected at higher perfusion pressures, underlining the compensatory role of vasomotor control mechanisms.
Authors:
Ozlem Yalcin; Herbert J Meiselman; Jonathan K Armstrong; Oguz K Baskurt
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biorheology     Volume:  42     ISSN:  0006-355X     ISO Abbreviation:  Biorheology     Publication Date:  2005  
Date Detail:
Created Date:  2005-12-21     Completed Date:  2006-06-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372526     Medline TA:  Biorheology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  511-20     Citation Subset:  IM    
Affiliation:
Department of Physiology, Akdeniz University Faculty of Medicine, Antalya, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological
Animals
Blood Sedimentation
Erythrocyte Aggregation / drug effects,  physiology*
Female
Guinea Pigs
Hematocrit
Hemorheology
Male
Muscle, Smooth, Vascular / physiology*
Papaverine / pharmacology
Perfusion
Regional Blood Flow
Vascular Resistance / physiology*
Vasoconstrictor Agents / pharmacology
Grant Support
ID/Acronym/Agency:
HL 70595/HL/NHLBI NIH HHS; HL15722/HL/NHLBI NIH HHS; IR03 TW01295/TW/FIC NIH HHS
Chemical
Reg. No./Substance:
0/Vasoconstrictor Agents; 58-74-2/Papaverine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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