| Effect of endotoxin on diaphragm lymph contamination in unanesthetized sheep. | |
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MedLine Citation:
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PMID: 3549676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The preparation for collecting lung lymph from sheep caudal mediastinal lymph node (CMN) efferent vessels is widely used to study the effects of endotoxin on lung microvascular permeability. However, there are nonpulmonary lymph vessels that drain into the CMN along with the afferent lymph vessels from the lung. Thus CMN lymph is a mixture of lymph from the lung and diaphragm lymph vessels as well as from other nonpulmonary lymph vessels. We studied the effect of 0.5-1.0 microgram/kg Escherichia coli endotoxin on the flow rates in diaphragm and CMN efferent lymph vessels (Qdi and QCMN, respectively) in unanesthetized sheep. For the time period between 2 and 5.5 h after endotoxin QCMN was increased from its base line of 7.2 +/- 4.4 (SD) to 17.3 +/- 10.6 ml/h and the lymph-to-plasma protein concentration ratio (L/PCMN) had increased from 0.68 +/- 0.11 to 0.81 +/- 0.06. During the same time period, Qdi was 4.5 +/- 3.1 ml/h compared with 1.0 +/- 0.8 ml/h at base line and the diaphragm lymph-to-plasma protein concentration ratio (L/Pdi) was 0.92 +/- 0.07 (base line = 0.74 +/- 0.15). The increases in flow rate and protein concentration were significant for each type of vessel (P less than 0.05). We conclude that the period of increased QCMN and L/PCMN after endotoxin is associated with an increase in Qdi and L/Pdi. Thus, it is difficult to determine how much of the CMN lymph response comes from the lungs and how much comes from diaphragm lymph vessels. |
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Authors:
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R E Drake; S J Allen; J C Gabel; J Katz; G A Laine |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 62 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 1987 Feb |
Date Detail:
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Created Date: 1987-05-18 Completed Date: 1987-05-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 706-10 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Diaphragm / metabolism* Endotoxins / pharmacology* Escherichia coli* Ink Lymph / metabolism* Proteins / metabolism Sheep |
| Grant Support | |
ID/Acronym/Agency:
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HL-27367/HL/NHLBI NIH HHS; HL-36115/HL/NHLBI NIH HHS; HL-36635/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Endotoxins; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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