| Effect of endothelin-1 on astrocytic protein content. | |
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MedLine Citation:
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PMID: 12730959 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The astrocytic endothelin (ET) receptors, ET(A) and ET(B), modulate calcium signaling and the astrocytic gap junctional network. The nonselective ET receptor ligand ET-1 inhibits gap junction permeability, an effect that can be blocked by tolbutamide. This mechanism may play a role in pathophysiological conditions such as ischemic stroke, characterized by elevated tissue ET-1 levels and hypertrophic-appearing reactive astrocytes. Therefore, the effect of ET-1 on cellular protein content was investigated in confluent once-passaged rat astrocyte cultures under serum-free conditions, by the Lowry method. Gap junction permeability was determined by the dye transfer technique. ET-1 prevented the decrease in astrocytic protein content observed in controls. The effect of ET-1 on cellular protein content was most pronounced in cultures seeded at high density, but it was attenuated in ET(B)-deficient (sl/sl) astrocytes. This effect could be blocked by the nonselective ET antagonist LU 302872 (10 micro M), as well as by the protein synthesis inhibitor cycloheximide (10 micro M). This increase in astrocytic protein content was inhibited by the ATP-sensitive K(+) channel blocker tolbutamide, which also antagonized the ET-1-induced reduction of gap junction permeability and reversed the morphological changes observed in astrocytes upon ET-1 treatment. Cytosine arabinoside (10 micro M), a DNA synthesis blocker, inhibited the ET-1-induced BrdU uptake without affecting the ET-1-induced increase in astrocytic protein content. To conclude, ET-1 induces an increase in astrocytic protein content as well as changes in astrocyte morphology in vitro. This hypertrophic response involves uncoupling of the astrocytic gap junctional network and is not dependent on DNA synthesis. |
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Authors:
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Martin Hasselblatt; Marion Bunte; Ralf Dringen; Arantxa Tabernero; José M Medina; Christian Giaume; Anna-Leena Sirén; Hannelore Ehrenreich |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Glia Volume: 42 ISSN: 0894-1491 ISO Abbreviation: Glia Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-05-05 Completed Date: 2003-08-04 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8806785 Medline TA: Glia Country: United States |
Other Details:
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Languages: eng Pagination: 390-7 Citation Subset: IM |
Copyright Information:
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Copyright 2003 Wiley-Liss, Inc. |
Affiliation:
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Max-Planck-Institut für Experimentelle Medizin, Göttingen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antimetabolites / pharmacokinetics Antimetabolites, Antineoplastic / pharmacology Astrocytes / cytology, drug effects*, metabolism* Bromodeoxyuridine / pharmacokinetics Cells, Cultured Cycloheximide / pharmacology Cytarabine / pharmacology Endothelin-1 / pharmacology* Gap Junctions / drug effects, physiology Hypoglycemic Agents / pharmacology Nerve Tissue Proteins / metabolism Propionic Acids / pharmacology Protein Synthesis Inhibitors / pharmacology Pyrimidines / pharmacology Rats Rats, Mutant Strains Rats, Wistar Receptors, Endothelin / antagonists & inhibitors, metabolism Tolbutamide / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antimetabolites; 0/Antimetabolites, Antineoplastic; 0/Endothelin-1; 0/Hypoglycemic Agents; 0/LU 224332; 0/Nerve Tissue Proteins; 0/Propionic Acids; 0/Protein Synthesis Inhibitors; 0/Pyrimidines; 0/Receptors, Endothelin; 147-94-4/Cytarabine; 59-14-3/Bromodeoxyuridine; 64-77-7/Tolbutamide; 66-81-9/Cycloheximide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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