Document Detail

Effect of endogenous prostaglandins on acetylcholine release from dog trachealis muscle.
MedLine Citation:
PMID:  3298196     Owner:  NLM     Status:  MEDLINE    
We used a radioenzymatic technique to measure effects of the prostaglandin synthesis inhibitor indomethacin and of exogenous prostaglandin E2 (PGE2) and prostaglandin I2 (PGI2) on acetylcholine (ACh) efflux from canine tracheal smooth muscle (TSM) during sustained electrical field stimulation (EFS; 2 Hz, 2 ms pulse duration, 50 V for 15 min). ACh efflux from indomethacin (INDO, 10(-6) M)-pretreated and control TSM increased with consecutive stimulations. However, efflux of ACh was greater in INDO-treated than control muscles. INDO increased the tension produced by TSM in response to EFS. Neither PGE2 (10(-8) M) nor PGI2 (10(-6) M) had any effect on ACh efflux from INDO-pretreated TSM during the first of three periods of EFS. However, PGI2 and PGE2 prevented the progressive increase in ACh efflux observed on subsequent stimulations. PGE2 but not PGI2 decreased contractions of TSM caused by EFS. Our results demonstrate that endogenous prostaglandins, probably PGE2, do inhibit EFS-evoked ACh release from canine TSM in vitro, but suggest that these prostaglandins modulate EFS-evoked contractions predominantly by postsynaptic mechanisms.
S Shore; B Collier; J G Martin
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  62     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1987 May 
Date Detail:
Created Date:  1987-08-04     Completed Date:  1987-08-04     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1837-44     Citation Subset:  IM    
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MeSH Terms
Acetylcholine / secretion*
Electric Stimulation
Epoprostenol / pharmacology
Indomethacin / pharmacology
Muscle Contraction / drug effects
Muscle, Smooth / drug effects,  physiology
Prostaglandins / physiology*
Prostaglandins E / pharmacology
Trachea / drug effects,  physiology*
Reg. No./Substance:
0/Prostaglandins; 0/Prostaglandins E; 35121-78-9/Epoprostenol; 363-24-6/Dinoprostone; 51-84-3/Acetylcholine; 53-86-1/Indomethacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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