Document Detail


Effect of diuretics, channel modulators and signal interceptors on contractility of the trabecular meshwork.
MedLine Citation:
PMID:  9176896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Measurements of isometric tension were performed on isolated trabecular meshwork (TM) and ciliary muscle (CM) strips of the bovine eye. Anterior segments of bovine eyes with well-preserved TM were perfused to measure outflow rate. (1) Bumetanide (10(-4) mol/l) and hydrochlorothiazide (10(-4) mol/l) did not change the contractility of TM and CM strips in the presence or absence of carbachol. Ethacrynic acid dose-dependently relaxed TM and CM strips precontracted either by carbachol or endothelin. (2) Cytochalasin D totally relaxed TM and CM strips precontracted by carbachol. The outflow rate almost doubled after application of cytochalasin D. (3) The effects of various modulators of K+ and Ca2+ channels are summarized. Carbachol and endothelin are postulated to modify nonselective cation channels. An effective blocker of nonselective cation channels (flufenamic acid) relaxed TM and CM precontracted by carbachol or endothelin. These relaxing effects were independent of the relaxing effects of ethacrynic acid and isosorbide dinitrate. (4) Evidence for the presence of various transporters and receptors in TM cells is summarized. (5) The TM per se is a contractile element which is involved in the regulation of aqueous humor outflow. The contractility of TM and CM cells is differently modulated.
Authors:
M Wiederholt; N Dörschner; J Groth
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde     Volume:  211     ISSN:  0030-3755     ISO Abbreviation:  Ophthalmologica     Publication Date:  1997  
Date Detail:
Created Date:  1997-08-11     Completed Date:  1997-08-11     Revised Date:  2007-09-17    
Medline Journal Info:
Nlm Unique ID:  0054655     Medline TA:  Ophthalmologica     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  153-60     Citation Subset:  IM    
Affiliation:
Institut für Klinische Physiologie, Universitätsklinikun Benjamin Franklin, Freie Universität Berlin, Germany. wiederho@zedat.fu-berlin.de
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MeSH Terms
Descriptor/Qualifier:
Absorption
Animals
Anterior Eye Segment / cytology,  drug effects,  physiology
Aqueous Humor / physiology
Bumetanide / pharmacology
Carbachol / pharmacology
Cattle
Cells, Cultured
Ciliary Body / cytology,  drug effects,  physiology
Cytochalasin D / pharmacology
Diuretics / pharmacology*
Ethacrynic Acid / pharmacology
Hydrochlorothiazide / pharmacology
Ion Channels / drug effects,  physiology*
Isometric Contraction / drug effects*
Muscle, Smooth / cytology,  drug effects,  metabolism
Parasympathomimetics / pharmacology*
Patch-Clamp Techniques
Perfusion
Signal Transduction / drug effects*
Trabecular Meshwork / cytology,  drug effects,  physiology*
Chemical
Reg. No./Substance:
0/Diuretics; 0/Ion Channels; 0/Parasympathomimetics; 22144-77-0/Cytochalasin D; 28395-03-1/Bumetanide; 51-83-2/Carbachol; 58-54-8/Ethacrynic Acid; 58-93-5/Hydrochlorothiazide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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