Document Detail


Effect of dimerization of a beta-turn antimicrobial peptide, PST13-RK, on antimicrobial activity and mammalian cell toxicity.
MedLine Citation:
PMID:  18815734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PST13-RK (KKKFPWWWPFKKK-NH(2)) is an improved derivative of tritrpticin adopting a beta-turn structure. In order to investigate the effect of dimerization of PST13-RK on antimicrobial activity and mammalian cell toxicity, we designed and synthesized its Cys- and Lys-linked dimers. The dimerization of PST13-RK resulted in a 2-4 fold decreased antimicrobial activity against Gram-positive and Gram-negative bacteria. However, the dimers showed a large increase in mammalian cell toxicity against mouse NIH-3T3, human MDA-MB-361, and human A549 cells. These results suggested that PST13-RK is active as a monomer to bacterial cells but as an oligomer to mammalian cells. Since the dimeric PST13-RK is much more effective against the cancer cells than the monomer, it might be an attractive candidate for anticancer chemotherapeutic drugs.
Authors:
Sung-Tae Yang; Jae Il Kim; Song Yub Shin
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Publication Detail:
Type:  Journal Article     Date:  2008-09-25
Journal Detail:
Title:  Biotechnology letters     Volume:  31     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2008-12-31     Completed Date:  2009-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  233-7     Citation Subset:  IM    
Affiliation:
Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / administration & dosage*,  chemistry*
Antimicrobial Cationic Peptides / administration & dosage*,  chemistry*
Apoptosis / drug effects*
Cell Survival / drug effects*
Dimerization
Humans
Mice
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Antimicrobial Cationic Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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