| Effect of different lysing and washing methods on free amino acid concentrations of sheep whole blood and erythrocytes. | |
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MedLine Citation:
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PMID: 954179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. A study was conducted to determine the effect of different washing and lysing methods on the free amino acid concentrations of sheep erythryocytes (RBC) and whole blood. Methods of lysis included laking, freeze-thawing or sonication. RBC were washed with isotonic solutions of either saline or sucrose. 2. Washing the RBC with isotonic sucrose resulted in significantly greater concentrations for ten of the amino acids. Greater amino acid concentrations were realized when washed RBC were sonicated and when the RBC of whole blood were lysed by laking. 3. Suggestions are made for future application to whole blood and RBC amino acid determinations. |
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Authors:
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G W Wolfrom; J M Asplund |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Clinical biochemistry Volume: 9 ISSN: 0009-9120 ISO Abbreviation: Clin. Biochem. Publication Date: 1976 Aug |
Date Detail:
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Created Date: 1976-11-01 Completed Date: 1976-11-01 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0133660 Medline TA: Clin Biochem Country: CANADA |
Other Details:
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Languages: eng Pagination: 180-3 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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blood* Animals Erythrocytes / analysis* Evaluation Studies as Topic Hemolysis Methods Sheep |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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