| Effect of different carbohydrates on in vitro fermentation activity and bacterial numbers of porcine inocula under osmotic stress conditions. | |
| | |
MedLine Citation:
|
PMID: 22256675 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
The bacteria of the intestine have to cope with varying osmotic conditions in their ecosystem. In this in vitro study, the modified Hohenheim gas test (HGT) was used to determine fermentation activity and bacterial composition of pig's faecal microbial inoculum, when fermenting different carbohydrates (inulin, corn starch, potato starch, cellulose, pectin), under normal buffered and osmotic stress conditions (elevated medium salinity). After 24 h of fermentation, gas, ammonia and short-chain fatty acid (SCFA) production was measured, and the cell numbers of total eubacteria, Lactobacillus spp., Bifidobacterium spp. and enterobacteria were analysed, using real-time polymerase chain reaction. There was a significant reduction in gas production after 24 h when comparing osmotic stress conditions with normal buffered conditions, and there were also differences among carbohydrates under both conditions. The content of SCFA was significantly lower when comparing osmotic stress with normal buffered conditions. Under osmotic stress, inulin and corn starch increased (p < 0.05) cell numbers of total eubacteria, while Bifidobacterium spp. and enterobacteria were higher (p < 0.05) when corn starch and pectin were fermented, respectively, in comparison to the other carbohydrates. The in vitro system of the modified HGT appears to be suitable to scrutinise effects of carbohydrates on the metabolic activity and composition of the microbial community under osmotic stress conditions, as they might occur during situations of osmotic diarrhoea. |
| | |
Authors:
|
Franziska Rink; Eva Bauer; Meike Eklund; Rainer Mosenthin |
Related Documents
:
|
12635705 - In vitro organogenesis and genetic transformation in popular cucumis sativus l. through... 93505 - Regulation of internal solute concentrations of marine vibrio alginolyticus in response... 20375425 - Enzymatic hydrolysis of trehalose dimycolate releases free mycolic acids during mycobac... 17192845 - Factors involved in anaerobic growth of saccharomyces cerevisiae. 11838975 - Chemistry of plants from crete: stachyspinoside, a new flavonoid glycoside and iridoids... 19882625 - A gc-magnetic sector ms method for identification and quantification of fatty acids in ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Archives of animal nutrition Volume: 65 ISSN: 1745-039X ISO Abbreviation: Arch Anim Nutr Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2012-01-19 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101222433 Medline TA: Arch Anim Nutr Country: England |
Other Details:
|
Languages: eng Pagination: 445-59 Citation Subset: IM |
Affiliation:
|
Institute of Animal Nutrition, University of Hohenheim, Stuttgart, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of a microencapsulated feed additive of lactic and formic acid on the prevalence of Salmonell...
Next Document: Effects of high dietary fibre diets formulated from by-products from vegetable and agricultural indu...