Document Detail

Effect of dietary protein on calpastatin in canine skeletal muscle.
MedLine Citation:
PMID:  12968694     Owner:  NLM     Status:  MEDLINE    
The cysteine proteinases, mu- and m-calpain, along with their inhibitor, calpastatin, have been hypothesized to play a role in skeletal muscle protein degradation. Because nutrition has previously been shown to influence the expression of calpastatin, the working hypothesis of this study was that the quantity and source of dietary protein could influence regulation of the calpain system in muscle. The objectives to support this hypothesis were to determine the effects of dietary protein (amount and source) on the expression of calpastatin in canine skeletal muscle. This study comprised eight diets with seven dogs per diet. A biopsy was taken from the biceps femoris of all 56 dogs before and after 10 wk on their respective diets. This experimental design allowed examination of change within individual dogs. Diets 1 to 4 contained 12% total protein derived from chicken and/or corn gluten meal in ratios of 100:0, 67:33, 33:67, and 0:100%, respectively. Diets 5 to 8 contained 28% total protein with protein sources and ratios identical to Diets 1 to 4. Differences in calpastatin were examined qualitatively using SDS-PAGE and immunoblotting, and semiquantitatively with densitometric analyses. The majority of the calpastatin blots showed three distinct calpastatin bands, the uppermost appearing at approximately 110 kDa. Diet 5 (28% CP, 100% chicken) resulted in an increase in the expression of the 110-kDa calpastatin band compared with the other two lower molecular weight bands in the same samples. Muscle from dogs fed Diet 5 showed greater increase in (P < 0.05) calpastatin intensity of the topmost band than those fed Diet 8 (0:100; chicken:corn gluten meal). Diet 5 (100:0; chicken:corn gluten meal) showed greater total calpastatin intensity than Diet 8 (0:100; chicken:corn gluten meal). These data suggest that dogs fed a diet containing a higher total percentage of chicken protein may have a greater potential to regulate calpain-mediated degradation of muscle protein than dogs fed diets containing corn gluten meal.
E E Helman; E Huff-Lonergan; G M Davenport; S M Lonergan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of animal science     Volume:  81     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-12     Completed Date:  2004-01-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2199-205     Citation Subset:  IM    
Animal Science Department, Iowa State University, Ames 50011, USA.
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MeSH Terms
Animal Feed
Animal Nutritional Physiological Phenomena
Biopsy / veterinary
Calcium-Binding Proteins / metabolism*
Cysteine Proteinase Inhibitors / metabolism*
Densitometry / veterinary
Dietary Proteins / administration & dosage,  metabolism,  pharmacology*
Dogs / growth & development,  metabolism*
Electrophoresis, Polyacrylamide Gel / veterinary
Immunoblotting / veterinary
Molecular Weight
Muscle, Skeletal / metabolism*,  pathology
Random Allocation
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/Cysteine Proteinase Inhibitors; 0/Dietary Proteins; 79079-11-1/calpastatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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