Document Detail


Effect of dietary meat and fish on endogenous nitrosation, inflammation and genotoxicity of faecal water.
MedLine Citation:
PMID:  20106932     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
N-3 polyunsaturated fatty acids have been associated with reduced colon tumorigenesis. However, their association with colorectal cancer incidence is not conclusive. We investigated the influence of isocaloric replacement of red meat with fatty fish on endogenous nitrosation, inflammation and genotoxicity of faecal water in apparently healthy human volunteers on controlled diets. Fourteen volunteers consumed a high red meat, a combined red meat/fish and a high fish diet for 8 days each. Faecal homogenates were analysed for haem, nitroso compounds (NOC) and calprotectin and associated supernatants for genotoxicity. Both faecal NOC and haem excretion decreased with more fish and less meat in the diet. Nitrosyl iron (FeNO) was the main contributor to total NOC on all diets. The proportion of other NOC increased with more fish and less meat in the diet (P = 0.01), resulting in a non-statistically significant decrease in the proportion of FeNO on the fish diet. There was no statistically significant difference in faecal calprotectin (P = 0.54) and faecal water-induced DNA strand breaks and oxidized purines and pyrimidines between the diets (P > 0.36). Increasing fish intake and reducing the intake of red meat does not seem to have an effect on inflammation and faecal water-induced (oxidative) DNA damage; however, it does reduce the formation of mutagenic and potentially carcinogenic NOC and may as such beneficially affect colorectal risk.
Authors:
Annemiek M C P Joosen; Emmanuelle Lecommandeur; Gunter G C Kuhnle; Sue M Aspinall; Lisanne Kap; Sheila A Rodwell
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't     Date:  2010-01-27
Journal Detail:
Title:  Mutagenesis     Volume:  25     ISSN:  1464-3804     ISO Abbreviation:  Mutagenesis     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-03     Completed Date:  2010-09-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8707812     Medline TA:  Mutagenesis     Country:  England    
Other Details:
Languages:  eng     Pagination:  243-7     Citation Subset:  IM    
Affiliation:
MRC Dunn Human Nutrition Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK. annemiek@srl.cam.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Animals
Biological Markers / metabolism
DNA Breaks
Diet*
Feces / chemistry*
Feeding Behavior
Female
Fishes*
Heme / metabolism
Humans
Inflammation / metabolism*
Male
Meat*
Middle Aged
Mutagens / analysis*
Nitrosation
Nitroso Compounds / metabolism
Water / chemistry*
Young Adult
Grant Support
ID/Acronym/Agency:
//Medical Research Council
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Mutagens; 0/Nitroso Compounds; 14875-96-8/Heme; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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