Document Detail


Effect of dietary conjugated linoleic acid on the fatty acid composition of egg yolk, plasma and liver as well as hepatic stearoyl-coenzyme A desaturase activity and gene expression in laying hens.
MedLine Citation:
PMID:  16479945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A total of 216 Brown Dwarf laying hens (1.62 +/- 0.06 kg BW and 60 wk old) were fed 1 of 3 corn-soybean meal-based diets containing 0, 2.5, or 5.0% conjugated linoleic aicd (CLA) to explore its effects on the fatty acid composition of egg yolk, plasma, and liver as well as hepatic stearoyl-coenzyme A desaturase-1 (SCD-1) activity and its mRNA gene expression. Four hens were placed in wired-floored cages (45 x 40 x 45 cm) and 3 cages were grouped as 1 replicate, resulting in 6 replicates per treatment. The experimental diets were fed for 54 d, and then eggs were collected to determine the fatty acid composition of egg yolk. Four eggs were randomly selected from the total day's production for each replicate, and the contents were pooled prior to analysis. On d 56, one randomly chosen hen from each replicate (6 hens per replicate and a total of 18 hens) was bled via heart puncture and then killed in order to collect liver samples to measure the fatty acid profile of plasma and liver tissue as well as hepatic SCD-1 activity and its mRNA abundance. Dietary supplementation of CLA resulted in a significant deposition of CLA in egg yolk, plasma, and liver lipids (P < 0.01). As the dietary level of CLA increased, the concentration of saturated fatty acids in egg yolk, plasma, and liver also increased (P < 0.05). However, the concentration of monounsaturated fatty acids in these same tissues decreased (P < 0.01). Compared with the control, the activity of SCD-1 was reduced by feeding 2.5% CLA (P < 0.05) without a change in SCD-1 mRNA gene expression. However, feeding 5% CLA reduced both SCD-1 activity and mRNA abundance (P < 0.05). These results indicate that the conversion of saturated to monounsaturated fatty acids in egg yolk, plasma, and liver might be modulated directly at hepatic mRNA gene expression levels, or may be indirectly regulated at the downstream post-transcriptional levels.
Authors:
X G Shang; F L Wang; D F Li; J D Yin; X J Li; G F Yi
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Publication Detail:
Type:  Clinical Trial; Journal Article    
Journal Detail:
Title:  Poultry science     Volume:  84     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2006-02-16     Completed Date:  2006-04-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1886-92     Citation Subset:  IM    
Affiliation:
National Key Lab of Animal Nutrition, China Agricultural University, Beijing, People's Republic of China 100094.
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Animals
Chickens
Diet
Dose-Response Relationship, Drug
Egg Yolk / chemistry*
Fatty Acids / analysis*,  blood*
Female
Gene Expression Regulation, Enzymologic / drug effects*
Linoleic Acids, Conjugated / administration & dosage,  pharmacology*
Liver / chemistry,  drug effects*,  enzymology
Stearoyl-CoA Desaturase / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Linoleic Acids, Conjugated; EC 1.14.19.1/Stearoyl-CoA Desaturase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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