Document Detail


Effect of dietary fatty acid composition on Th1/Th2 polarization in lymphocytes.
MedLine Citation:
PMID:  19221048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: It has become increasingly clear that polyunsaturated fatty acids (PUFAs) have immunomodulatory effects. However, the intake of these fatty acids used in animal studies often greatly exceeds dietary human intake. Whether differences in the composition of fatty acids that are consumed in amounts consistent with normal dietary intake can influence immune function remains uncertain. METHODS: We manufactured 3 types of liquid diet, related to modified fatty acid composition (omega-6/omega-3 = 0.25, 2.27 and 42.9), but excluding eicosapentaenoic acid and docosahexaenoic acid, based upon a liquid diet used clinically in humans. We assessed CD3-stimulated cytokine production of splenocytes in female BALB/c mice (n = 4 per group) fed 1 of 3 liquid diets for 4 weeks. We also measured the cytokine production of peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin in humans at the end of a 4-week period of consumption of 2 different liquid diets (omega-6/omega-3 = 3 and 44). RESULTS: We found that the ratio of interfero omega-gamma (IFN-gamma) / interleukin-4 (IL-4) was significantly higher in mice fed the omega-3 rich diet than in others. In humans, IFN-gamma / IL-4 was significantly higher after the omega-3 versus the omega-6 enhanced diet. CONCLUSIONS: Differences in the composition of omega-3 and omega-6 PUFAs induces a shift in the Th1/Th2 balance in both mouse and human lymphocytes, even when ingested in normal dietary amounts. An omega-3 rich diet containing alpha-linolenic acid modulates immune function.
Authors:
Takamitsu Mizota; Chiaki Fujita-Kambara; Nemu Matsuya; Shinji Hamasaki; Takayasu Fukudome; Hirofumi Goto; Shunya Nakane; Takayuki Kondo; Hidenori Matsuo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-12
Journal Detail:
Title:  JPEN. Journal of parenteral and enteral nutrition     Volume:  33     ISSN:  0148-6071     ISO Abbreviation:  JPEN J Parenter Enteral Nutr     Publication Date:    2009 Jul-Aug
Date Detail:
Created Date:  2009-06-12     Completed Date:  2009-08-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804134     Medline TA:  JPEN J Parenter Enteral Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  390-6     Citation Subset:  IM    
Affiliation:
Department of Clinical Neurosciences, Nagasaki University Graduate School of Biomedical Sciences, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Animals
Dietary Fats / pharmacology*
Enteral Nutrition
Enzyme-Linked Immunosorbent Assay
Fatty Acids, Unsaturated / pharmacology*
Female
Humans
Interferon-gamma / blood
Interleukin-4 / blood
Male
Mice
Mice, Inbred BALB C
Middle Aged
T-Lymphocytes / drug effects*,  immunology
Th1 Cells*
Th2 Cells*
Chemical
Reg. No./Substance:
0/Dietary Fats; 0/Fatty Acids, Unsaturated; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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