Document Detail

Effect of diet-induced hypercholesterolemia on proteoglycan metabolism in guinea pig megakaryocytes and platelets.
MedLine Citation:
PMID:  1987997     Owner:  NLM     Status:  MEDLINE    
Proteoglycan metabolism was evaluated in megakaryocytes and platelets from guinea pigs fed a 1% cholesterol diet for 3 or 7 weeks. The animals were injected with a single dose of [35S]sulfate at the end of the feeding period, and megakaryocytes and platelets were isolated after 3 hours and then daily for 4 days thereafter. Proteoglycans were extracted from the cells of each animal and analyzed by ion-exchange chromatography, gel filtration, and electrophoresis. The maximal labeling of platelets occurred 2 days after [35S]sulfate injection as compared with 3 days in controls. A proteoglycan that eluted at Kav 0.2 from the Sepharose CL-6B column appeared 1 day after labeling. Additional proteoglycans of Kav 0.15 appeared at subsequent time points. The labeling profile for cholesterol-fed animals was unchanged from 2-4 days, unlike profiles from controls, which had exhibited a gradual increase in mean proteoglycan size. Thus, the progressive change in size of proteoglycans synthesized during normal megakaryocyte maturation was altered. The mean chain length of the proteoglycan-associated glycosaminoglycans from cholesterol-fed animals was increased relative to that of controls. In conjunction with the twofold increase in mean megakaryocyte size induced by cholesterol feeding in guinea pigs, the changes in proteoglycan synthesis suggest a state of stimulated megakaryocytopoiesis.
B P Schick
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Arteriosclerosis and thrombosis : a journal of vascular biology / American Heart Association     Volume:  11     ISSN:  1049-8834     ISO Abbreviation:  Arterioscler. Thromb.     Publication Date:    1991 Jan-Feb
Date Detail:
Created Date:  1991-02-27     Completed Date:  1991-02-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9101388     Medline TA:  Arterioscler Thromb     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  191-7     Citation Subset:  IM    
Cardeza Foundation for Hematologic Research, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pa.
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MeSH Terms
Blood Platelets / metabolism*
Cholesterol, Dietary / administration & dosage
Chromatography, Ion Exchange
Guinea Pigs
Hypercholesterolemia / metabolism*
Megakaryocytes / metabolism*
Molecular Weight
Proteoglycans / metabolism*
Grant Support
Reg. No./Substance:
0/Cholesterol, Dietary; 0/Proteoglycans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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