Document Detail

Effect of dichlorvos on the acetylcholinesterase from tambaqui (Colossoma macropomum) brain.
MedLine Citation:
PMID:  17665685     Owner:  NLM     Status:  MEDLINE    
Dichlorvos is an acutely toxic organophosphorous pesticide that is known as a classical acetylcholinesterase (AChE; EC inhibitor. Here, the brain AChE from the important Amazonian fish tambaqui (Colossoma macropomum) was assayed in the presence of this insecticide and also of deltamethrin, a classical sodium and potassium channel inhibitor (negative control). Four tissue homogenates were analyzed in triplicate for AChE activity using acetylthiocholine as the substrate and 5,5'-dithiobis(2-nitrobenzoic) acid (DTNB) as the color-developing agent. Each tissue homogenate represented pooled brains from five fish. The inhibitory effect of dichlorvos on AChE activities was determined at concentrations from 0.001 to 10 ppm and compared to controls. This effect followed an exponential decay model (y = 9.420 + 26.192e(-x/5.380); r2 = 0.989), presenting IC50 (the concentration of dichlorvos that is required for 50% of AChE inhibition) of 0.081 ppm (0.368 micromol/L). No effect was observed for the deltamethrin, and the concentration 0.0452 micromol/L of dichlorvos was significantly different from this control. These results suggest that tambaqui brain AChE can be proposed as a biomarker for dichlorvos and can be used as a tool for aquatic environment monitoring.
Caio Rodrigo Dias Assis; Ian Porto Gurgel Amaral; Patrícia Fernandes Castro; Luiz Bezerra Júnior Carvalho; Ranilson Souza Bezerra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental toxicology and chemistry / SETAC     Volume:  26     ISSN:  0730-7268     ISO Abbreviation:  Environ. Toxicol. Chem.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-08-01     Completed Date:  2007-09-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8308958     Medline TA:  Environ Toxicol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1451-3     Citation Subset:  IM    
Laboratório de Enzimologia-LABENZ, Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife-PE, Brazil.
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MeSH Terms
Acetylcholinesterase / drug effects*
Brain / drug effects*,  enzymology
Dichlorvos / toxicity*
Environmental Monitoring / methods
Pesticides / toxicity*
Water Pollutants, Chemical / toxicity*
Reg. No./Substance:
0/Pesticides; 0/Water Pollutants, Chemical; 62-73-7/Dichlorvos; EC

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