Document Detail


Effect of dichloromethylene diphosphonate (Cl2-MDP) on bone resorption induced by PHA-stimulated human mononuclear cells in organ culture.
MedLine Citation:
PMID:  6236769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human mononuclear cells stimulated by PHA caused resorption of fetal rat bones in tissue culture. Non-adherent cells alone failed to produce substantial bone resorbing activity. The mixed culture of adherent and non-adherent cells (1:40) produced significant bone mineral resorption in vitro. The bone resorption induced by human mononuclear cells stimulated by PHA required the presence of living bone tissue in culture. The bone resorbing effect caused by PHA-stimulated cells was blocked by dichloromethylene diphosphonate at 10(-5) M concentration, but mononuclear cells preincubated in diphosphonate containing media were capable to resorb fetal rat bone in tissue culture.
Authors:
H M Holak; S Szymaniec; A Skriabin
Related Documents :
8582079 - In vitro production of parathyroid hormone-related protein by cholesteatoma and normal ...
8838279 - Human osteoclast-like cells in primary culture.
3236209 - Direct regulation of bone resorption of chick embryonic femur in organ culture by the c...
8964519 - Immunohistochemical detection of activin a in osteoclasts.
11067809 - Rapid screening method for osteoclast differentiation in vitro that measures tartrate-r...
1564429 - Osteoclast formation together with interleukin-6 production in mouse long bones is incr...
7961139 - Ultrastructural features of composite skin cultures grafted onto athymic mice.
7527059 - Development of a spontaneous permanent cell line of rabbit corneal epithelial cells tha...
16348329 - Evidence that cellulolysis by an anaerobic ruminal fungus is catabolite regulated by gl...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archivum immunologiae et therapiae experimentalis     Volume:  32     ISSN:  0004-069X     ISO Abbreviation:  Arch. Immunol. Ther. Exp. (Warsz.)     Publication Date:  1984  
Date Detail:
Created Date:  1984-10-12     Completed Date:  1984-10-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0114365     Medline TA:  Arch Immunol Ther Exp (Warsz)     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  77-83     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bone Resorption / drug effects*
Cell Adhesion
Clodronic Acid / pharmacology*
Diphosphonates / pharmacology*
Humans
Leukocytes / immunology*
Lymphocyte Activation
Lymphokines / biosynthesis
Organ Culture Techniques
Phytohemagglutinins / pharmacology
Rats
Chemical
Reg. No./Substance:
0/Diphosphonates; 0/Lymphokines; 0/Phytohemagglutinins; 10596-23-3/Clodronic Acid; 64060-24-8/osteoclast activating factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cardiac relaxation in experimental chronic myocardial hypertrophy: role of the sarcoplasmic reticulu...
Next Document:  An unusual kind of muscular and skeletal involvement in a heroin addict. A case report.