| Effect of diabetes on rat liver cytochrome P-450. Evidence for a unique diabetes-dependent rat liver cytochrome P-450. | |
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MedLine Citation:
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PMID: 7150359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Detergent-solubilized hepatic microsomal fractions from alloxan diabetic rats exhibited a 52,000 molecular weight hemeprotein band that was not present in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein profiles of identically solubilized hepatic microsomal fractions from normal, 3-methylcholanthrene- or phenobarbital-treated rats. This 52,000 mol. wt hemeprotein band disappeared from the protein profile of insulin-treated diabetic rat liver to yield the SDS-PAGE profile of normal rat liver. When P-450 hemeproteins were purified by lauric acid affinity and hydroxylapatite chromatography from solubilized microsomes, only the diabetic rat had a 52,000 mol. wt P-450. This distinct 52,000 mol. wt diabetes-induced P-450 interacted with type II compounds to yield a 2-fold greater absorbance change than was observed with the purified P-450s from either the normal or the chemically induced rats. The properties of this unique 52,000 mol. wt P-450 suggest that it may be the catalytic component responsible for the increased rate of type II substrate (aniline) metabolism observed in the diabetic rat. |
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Authors:
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M R Past; D E Cook |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical pharmacology Volume: 31 ISSN: 0006-2952 ISO Abbreviation: Biochem. Pharmacol. Publication Date: 1982 Oct |
Date Detail:
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Created Date: 1983-01-19 Completed Date: 1983-01-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0101032 Medline TA: Biochem Pharmacol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 3329-34 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aminopyrine
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metabolism Aniline Compounds / metabolism Animals Cytochrome P-450 Enzyme System / isolation & purification, metabolism* Diabetes Mellitus, Experimental / enzymology* Liver / enzymology* Male Mitochondria, Liver / enzymology Proadifen / pharmacology Rats Rats, Inbred Strains Substrate Specificity |
| Grant Support | |
ID/Acronym/Agency:
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AM 21282/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aniline Compounds; 302-33-0/Proadifen; 58-15-1/Aminopyrine; 62-53-3/aniline; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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