| Effect of desmopressin acetate on hindlimb perfusion pressure in rats: what is the mechanism? | |
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MedLine Citation:
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PMID: 1510263 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Desmopressin acetate (DDAVP) is a synthetic analogue of vasopressin used to promote hemostasis and reduce postoperative blood loss. Desmopressin acetate can cause hypotension in humans. Our study evaluated the hemodynamics of rapid administration of DDAVP into the isolated hindlimb in live rats and assessed this response after pretreatment with various antagonists. Thirty male Sprague-Dawley rats (350-450 g) were given intraperitoneal pentobarbital anesthesia (50 mg/kg). Perfusion was set at a rate that gave a control mean hindlimb perfusion pressure (HPP) of 100-120 mm Hg. Rats were assigned to five groups (N = 5, each group), with each rat serving as its own control. As a control, saline solution (in volumes equivalent to those used for the antagonists) was injected into the hindlimb preparation before the agonist injections. Each group received both the clinical preparation of DDAVP (i.e., with preservative) and a laboratory preparation of DDAVP in doses of 0.3-3 ng. Group 1 was tested before and after injection of saline solution control; group 2, before and after propranolol (0.5 mg/kg); group 3, before and after meclofenamate (1.5 mg/kg), a cyclooxygenase inhibitor; group 4, before and after nitroarginine (5 mg/kg) an inhibitor of nitric oxide synthesis; and group 5, before and after atropine sulfate (1 mg/kg). Chlorobutanol (25-75 micrograms), the preservative in the clinical preparation of DDAVP, was tested for changes in HPP in five rats similarly prepared. Systemic mean arterial pressure remained constant during the study. The HPP decreased with increasing doses of the clinical preparation of DDAVP, compared with saline solution controls, whereas no change occurred with the laboratory preparation of DDAVP.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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J S Jahr; R H Holton; C J Feng |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Anesthesia and analgesia Volume: 75 ISSN: 0003-2999 ISO Abbreviation: Anesth. Analg. Publication Date: 1992 Sep |
Date Detail:
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Created Date: 1992-09-22 Completed Date: 1992-09-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1310650 Medline TA: Anesth Analg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 411-5 Citation Subset: AIM; IM |
Affiliation:
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Department of Anesthesiology, Tulane University School of Medicine, New Orleans, LA 70112. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anesthesia Animals Arginine / analogs & derivatives, pharmacology Atropine / pharmacology Blood Pressure / drug effects Chlorobutanol / pharmacology Deamino Arginine Vasopressin / pharmacology* Dose-Response Relationship, Drug Hindlimb / blood supply*, drug effects Male Meclofenamic Acid / pharmacology Nitric Oxide / antagonists & inhibitors Nitroarginine Perfusion Propranolol / pharmacology Rats Rats, Inbred Strains Vascular Resistance / drug effects |
| Chemical | |
Reg. No./Substance:
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10102-43-9/Nitric Oxide; 16679-58-6/Deamino Arginine Vasopressin; 2149-70-4/Nitroarginine; 51-55-8/Atropine; 525-66-6/Propranolol; 57-15-8/Chlorobutanol; 644-62-2/Meclofenamic Acid; 74-79-3/Arginine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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