Document Detail

Effect of deprival of LH on Leydig cell proliferation: involvement of PCNA, cyclin D3 and IGF-1.
MedLine Citation:
PMID:  10854704     Owner:  NLM     Status:  MEDLINE    
The levels of proliferating cell nuclear antigen (PCNA) and cyclin D3 which are known markers of cellular proliferation were monitored by immunoblotting in progenitor Leydig cells (PLC), immature Leydig cells (ILC) and adult Leydig cells (ALC) isolated from 21, 35 and 90 day old rats, respectively which represent the Leydig cells at different stages of development. The levels of PCNA and cyclin D3 were highest in PLC, intermediate in ILC and lowest in ALC. Following administration of an antiserum to LH to deprive endogenous LH in 21 day old rats, a significant decrease in the levels of PCNA and Cyclin D3 were observed suggesting the involvement of Lutenizing hormone (LH) in PLC proliferation. In support of this observation, Bromodeoxyuridine (BrdU) incorporation was highest in PLC when compared with ILC and ALC, and administration of LH antiserum to 21 day old rats led to a total absence of BrdU incorporation by the isolated PLC. Also, there was a decrease in the level of IGF-1 and IGF-1 receptor mRNA levels by 55 and 35%, respectively as assessed by semi-quantitative RT-PCR. In addition, the PLC isolated from rats deprived of endogenous LH incorporated much less BrdU following addition of IGF-1. These results, which are obtained using an in vivo model system establish that LH has a very important role in Leydig cell proliferation in immature rats.
V Sriraman; V S Rao; M R Sairam; A J Rao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  162     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-08-28     Completed Date:  2000-08-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  113-20     Citation Subset:  IM    
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
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MeSH Terms
Base Sequence
Cell Division / drug effects
Chorionic Gonadotropin / pharmacology
Cyclin D3
Cyclins / metabolism*
DNA Primers / genetics
Insulin-Like Growth Factor I / genetics,  metabolism*
Leydig Cells / cytology*,  drug effects,  metabolism*
Luteinizing Hormone / antagonists & inhibitors,  metabolism*
Proliferating Cell Nuclear Antigen / metabolism*
Rats, Wistar
Receptor, IGF Type 1 / genetics,  metabolism
Testis / cytology,  growth & development,  metabolism
Reg. No./Substance:
0/Ccnd3 protein, rat; 0/Chorionic Gonadotropin; 0/Cyclin D3; 0/Cyclins; 0/DNA Primers; 0/Proliferating Cell Nuclear Antigen; 67763-96-6/Insulin-Like Growth Factor I; 9002-67-9/Luteinizing Hormone; EC, IGF Type 1

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