Document Detail

Effect of dantrolene on lipid peroxidation, lutathione and glutathione-dependent enzyme activities in experimental otitis media with effusion in guinea pigs.
MedLine Citation:
PMID:  16323573     Owner:  NLM     Status:  MEDLINE    
The aims of our study were to assess whether the increased oxidative stress in experimental otitis media with effusion (OME) induced by histamine was reflected erythrocytes and middle ear effusion fluid by lipid peroxidation; to survey the alterations in antioxidant enzyme activities in experimental OME; and to determine the effect of dantrolene on this oxidative stress. Erythrocyte and middle ear effusion malondialdehyde (MDA) level, erythrocyte glutathione (GSH) and glutathione peroxidase (GSH-Px), glutathione reductase (GRD) and glutathione-S-transferase (GST) activities were measured in three groups of seven guinea pigs, 3 hours after injection of 0.1 mL of histamine (or saline) into the middle ear in guinea pigs with OME (experimental group), in a dantrolene sodium group and in a control group. Erythrocyte and effusion MDA levels in the dantrolene group were significantly lower than those of the experimental group. Erythrocyte GSH-Px, GST, GRD activities, and GSH levels were significantly higher in the dantrolene group than in the experimental group. Dantrolene sodium decreased the erythrocyte and effusion MDA levels, on the other hand, it increased the GSH and GSH-dependent enzymes. These findings suggest that reactive oxygen species (ROS) play a role in histamine-induced OME. Pre-treatment with dantrolene sodium increases antioxidant enzymes activities and decreases formation of MDA, the indicator of lipid peroxidation, in histamine-induced OME.
H Ucuncu; S Taysi; B Aktan; M E Buyukokuroglu; M Elmastas
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human & experimental toxicology     Volume:  24     ISSN:  0960-3271     ISO Abbreviation:  Hum Exp Toxicol     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-12-05     Completed Date:  2006-02-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9004560     Medline TA:  Hum Exp Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  567-71     Citation Subset:  IM    
Department of Otorhinolaryngology-Head and Neck Surgery, Ataturk University, Medical School, Erzurum, Turkey.
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MeSH Terms
Dantrolene / pharmacology*
Disease Models, Animal
Erythrocytes / drug effects*,  enzymology
Glutathione / metabolism
Glutathione Peroxidase / metabolism
Glutathione Reductase / metabolism
Glutathione Transferase / metabolism
Guinea Pigs
Lipid Peroxidation / drug effects*
Malondialdehyde / metabolism
Muscle Relaxants, Central / pharmacology*
Otitis Media with Effusion / chemically induced,  metabolism,  prevention & control*
Oxidative Stress / drug effects
Reg. No./Substance:
0/Muscle Relaxants, Central; 51-45-6/Histamine; 542-78-9/Malondialdehyde; 70-18-8/Glutathione; 7261-97-4/Dantrolene; EC Peroxidase; EC Reductase; EC Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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