Document Detail


Effect of cytokines on HLA-DR and IL-1 production by a monocytic tumour, THP-1.
MedLine Citation:
PMID:  2494105     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The monocytic tumour, THP-1, expresses many of the properties of monocytes, both by cell surface staining and its capacity to produce monokines. It was used as a source of homogenous monocytic cells as a model to determine whether a variety of highly purified or recombinant cytokines could induce HLA-DR expression and the production of interleukin-1 (IL-1). Interferon-gamma (IFN-gamma) alone induced HLA-DR. Tumour necrosis factor (TNF), lymphotoxin (LT) and granulocyte-macrophage colony-stimulating factor (GM-CSF) alone were able to induce IL-1 but not HLA-DR. When IFN-gamma was combined with TNF, induction of HLA-DR and IL-1 was enhanced in a synergistic manner. These effects were detectable at a pretranslational level as synergistic effects were observed on DR alpha mRNA and IL-1 beta mRNA levels. The results demonstrate the specificity of IFN-gamma as the inductive stimulus for HLA-DR expression by THP-1 cells. As IFN-gamma and TNF are products of activated T cells, the synergistic role for these molecules in macrophage activation is discussed.
Authors:
G Portillo; M Turner; D Chantry; M Feldmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  66     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-05-02     Completed Date:  1989-05-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  170-5     Citation Subset:  IM    
Affiliation:
Charing Cross Sunley Research Centre, Hammersmith, London.
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MeSH Terms
Descriptor/Qualifier:
Biological Factors / pharmacology*
Cytokines
HLA-DR Antigens / biosynthesis*
Humans
Interferon-gamma / pharmacology
Interleukin-1 / biosynthesis*
Leukemia, Monocytic, Acute / immunology*
Lymphotoxin-alpha / pharmacology
Tumor Cells, Cultured / drug effects,  immunology
Tumor Necrosis Factor-alpha / pharmacology
Chemical
Reg. No./Substance:
0/Biological Factors; 0/Cytokines; 0/HLA-DR Antigens; 0/Interleukin-1; 0/Lymphotoxin-alpha; 0/Tumor Necrosis Factor-alpha; 82115-62-6/Interferon-gamma
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