Document Detail


Effect of cyclosporine on apoptosis in bronchial epithelial cells.
MedLine Citation:
PMID:  22564603     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVES: Among airway complications, posttransplantation infections are related to impaired mucociliary clearance, which may represent a toxicity of cyclosporine (CsA), a potent, widely used immunosuppressive drug after organ transplantations. Since several recent studies have demonstrated CsA treatment to directly induce apoptosis in several cell types, we investigated its effects on airway cells using the human bronchial epithelial cell line BEAS-2B.
METHODS: Proliferation was measured by using a Cell Counting Assay Kit by exposing cells to CsA (0, 10, 30, 50, or 100 μg/mL). Apoptotic cells were identified using fluorescence microscopy after 4', 6-diamidino-2-phenylidole (DAPI) staining. Western blot analysis was performed to evaluate the contents of poly(adenosine diphosphate-ribose) polymerase (PARP), p27, Bcl-2, and caspase-3.
RESULTS: Cell viability decreased dependent on the CsA concentration: 100.00 ± 0.01% with 0 μg CsA as control; 98.65 ± 0.02% with 10 μg (P < .05 vs control); 95.41 ± 0.05% with 30 μg (P < .05 vs control); 38.84 ± 0.04% (P < .001 vs control) with 50 μg; and 15.28 ± 0.05% with 100 μg (P < .001 vs control). Apoptotic cells detected with DAPI showed chromatin condensation and nuclear fragmentation. CsA induced p27 and p53, as well as degradation of 116-kd PARP into an 89-kd fragment.
CONCLUSION: CsA induced apoptosis in human bronchial epithelial cells.
Authors:
E Y Ha; K C Mun
Related Documents :
16666523 - Water transport properties of cortical cells in roots of nitrogen- and phosphorus-defic...
11314343 - Actions of bovine plasma fibronectin on cultured human dental pulp cells.
20702363 - A method for accelerating progression to a malignant phenotype in rodent cells.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transplantation proceedings     Volume:  44     ISSN:  1873-2623     ISO Abbreviation:  Transplant. Proc.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0243532     Medline TA:  Transplant Proc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  985-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Kidney Institute, Keimyung University, Daegu, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Apoptosis in endothelial cells by cyclosporine.
Next Document:  Effects of cyclosporine on oxidative stress in human bronchial epithelial cells.