Document Detail


Effect of cyclophosphamide on the binding of 99mTcO-4 and 99mTc-MDP to blood cells and plasma proteins.
MedLine Citation:
PMID:  7581022     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since the introduction of technetium-99m (99mTc) and its rapid acceptance as a tool in nuclear medicine, very little information is available about its biological action as 99mTc-radiopharmaceuticals. We have determined if cyclophosphamide, an alkylating agent, used in oncology as a chemotherapeutic drug, modifies the binding of 99mTcO-4 and 99mTc-MDP (99mTc-methylenediphosphonic acid) to blood cells and to plasma proteins. The radiopharmaceuticals were injected intravenously (iv) into SW-55 mice (male and female, weight 25 g) and samples of plasma and blood cells were separated. Cyclophosphamide (50 micrograms) was injected iv 1 h before the radiopharmaceuticals. Samples of plasma and blood cells were also precipitated with 5% trichloroacetic acid and soluble and insoluble fractions were isolated. The following results were obtained: 1) cyclophosphamide did not alter (0.25 to 8 h) percent radioactivity of 99mTcO-4 in plasma or blood cells but increased the binding of 99mTc-MDP to blood cells; 2) cyclophosphamide did not alter (0.25 to 8 h) the binding of 99mTcO-4 in insoluble fraction of plasma and decreased (1 to 4 h) percent radioactivity of 99mTc-MDP in the insoluble fraction of plasma; 3) cyclophosphamide increased (0.25 to 4 h) percent radioactivity of 99mTcO-4 in the insoluble fraction of blood cells but did not alter the binding of 99mTc-MDP. Cyclophosphamide and/or its metabolites modified the effective half-life of these radiopharmaceuticals (to 99mTcO-4 was increased 2.3 to 3.4 h and to 99mTc-MDP was decreased 3.3 to 2.1 h) and possibly increased the permeability of blood cells to 99mTcO-4.
Authors:
J S Santos; E F de-Paula; T G Correa; L C de-Freitas; L M da-Fonseca; B Gutfilen; M Bernardo-Filho
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]     Volume:  28     ISSN:  0100-879X     ISO Abbreviation:  Braz. J. Med. Biol. Res.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-11-24     Completed Date:  1995-11-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112917     Medline TA:  Braz J Med Biol Res     Country:  BRAZIL    
Other Details:
Languages:  eng     Pagination:  131-5     Citation Subset:  IM    
Affiliation:
Instituto de Física, Universidade do Estado do Rio de Janeiro, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Alkylating Agents / pharmacology*
Animals
Blood Cells / drug effects*,  metabolism
Blood Proteins / drug effects*,  metabolism
Cyclophosphamide / pharmacology*
Female
Male
Mice
Sodium Pertechnetate Tc 99m / metabolism*
Technetium Tc 99m Medronate / metabolism*
Chemical
Reg. No./Substance:
0/Alkylating Agents; 0/Blood Proteins; 23288-60-0/Sodium Pertechnetate Tc 99m; 50-18-0/Cyclophosphamide; 63347-66-0/Technetium Tc 99m Medronate

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