| Effect of cyclophosphamide on the binding of 99mTcO-4 and 99mTc-MDP to blood cells and plasma proteins. | |
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MedLine Citation:
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PMID: 7581022 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Since the introduction of technetium-99m (99mTc) and its rapid acceptance as a tool in nuclear medicine, very little information is available about its biological action as 99mTc-radiopharmaceuticals. We have determined if cyclophosphamide, an alkylating agent, used in oncology as a chemotherapeutic drug, modifies the binding of 99mTcO-4 and 99mTc-MDP (99mTc-methylenediphosphonic acid) to blood cells and to plasma proteins. The radiopharmaceuticals were injected intravenously (iv) into SW-55 mice (male and female, weight 25 g) and samples of plasma and blood cells were separated. Cyclophosphamide (50 micrograms) was injected iv 1 h before the radiopharmaceuticals. Samples of plasma and blood cells were also precipitated with 5% trichloroacetic acid and soluble and insoluble fractions were isolated. The following results were obtained: 1) cyclophosphamide did not alter (0.25 to 8 h) percent radioactivity of 99mTcO-4 in plasma or blood cells but increased the binding of 99mTc-MDP to blood cells; 2) cyclophosphamide did not alter (0.25 to 8 h) the binding of 99mTcO-4 in insoluble fraction of plasma and decreased (1 to 4 h) percent radioactivity of 99mTc-MDP in the insoluble fraction of plasma; 3) cyclophosphamide increased (0.25 to 4 h) percent radioactivity of 99mTcO-4 in the insoluble fraction of blood cells but did not alter the binding of 99mTc-MDP. Cyclophosphamide and/or its metabolites modified the effective half-life of these radiopharmaceuticals (to 99mTcO-4 was increased 2.3 to 3.4 h and to 99mTc-MDP was decreased 3.3 to 2.1 h) and possibly increased the permeability of blood cells to 99mTcO-4. |
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Authors:
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J S Santos; E F de-Paula; T G Correa; L C de-Freitas; L M da-Fonseca; B Gutfilen; M Bernardo-Filho |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.] Volume: 28 ISSN: 0100-879X ISO Abbreviation: Braz. J. Med. Biol. Res. Publication Date: 1995 Jan |
Date Detail:
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Created Date: 1995-11-24 Completed Date: 1995-11-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8112917 Medline TA: Braz J Med Biol Res Country: BRAZIL |
Other Details:
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Languages: eng Pagination: 131-5 Citation Subset: IM |
Affiliation:
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Instituto de Física, Universidade do Estado do Rio de Janeiro, Brasil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkylating Agents
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pharmacology* Animals Blood Cells / drug effects*, metabolism Blood Proteins / drug effects*, metabolism Cyclophosphamide / pharmacology* Female Male Mice Sodium Pertechnetate Tc 99m / metabolism* Technetium Tc 99m Medronate / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Alkylating Agents; 0/Blood Proteins; 23288-60-0/Sodium Pertechnetate Tc 99m; 50-18-0/Cyclophosphamide; 63347-66-0/Technetium Tc 99m Medronate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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