Document Detail


Effect of cyclin G2 on proliferative ability of SGC-7901 cell.
MedLine Citation:
PMID:  15112359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To study the effect of cyclin G2 on proliferation of gastric adenocarcinoma cell line-SGC-7901 cell in vitro. METHODS: By use of cation lipofectamine transfection reagent, the pIRES-G2 and pIRESneo plasmids were transferred into SGC-7901cell line. Anticlones were selected by G418. Positive clones were observed and counted using Giemsa staining. Cell proliferative ability was assayed by MTT. RESULTS: (1) The clone number of pIRES-G2 group decreased, clone volume reduced. The number of cell clones in pIRESneo group was 87+/-3, that of pIRES-G2 group was 53+/-4, occupying 60.1% of pIRESneo group, there was significant difference obviously (P<0.01, t=15.45). (2) The average absorbance of clone cell obtained by stable transfection of pIRES-G2 at 570 nm was 1.6966+/-0.2125, the average absorbance of clone cell obtained by stable transfection of pIRESneo at 570 nm was 2.1182+/-0.3675, there was significant difference between them (P<0.01, t=3.412). CONCLUSION: Cyclin G2 can inhibit SGC-7901cell proliferative ability obviously, it may be a negative regulator in cell cycle regulation.
Authors:
Jie Liu; Ze-Shi Cui; Yang Luo; Li Jiang; Xiao-Hui Man; Xue Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  10     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-27     Completed Date:  2004-10-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  1357-60     Citation Subset:  IM    
Affiliation:
Experimental Technology Center, China Medical University, Shenyang 110001, Liaoning Province, China.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / metabolism
Cell Cycle / physiology
Cell Division / physiology*
Cell Line, Tumor
Cyclin G2
Cyclins / genetics,  metabolism*
Humans
Stomach Neoplasms / metabolism
Transfection
Chemical
Reg. No./Substance:
0/CCNG2 protein, human; 0/Cyclin G2; 0/Cyclins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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