Document Detail

Effect of cyclic mechanical stretch and titanium particles on prostaglandin E2 production by human macrophages in vitro.
MedLine Citation:
PMID:  14762933     Owner:  NLM     Status:  MEDLINE    
Early implant instability has been proposed as a critical factor in the onset and progression of aseptic loosening and periprosthetic osteolysis in total joint arthroplasties. Previous in vitro studies have reported that macrophages stimulated with cyclic mechanical strain release inflammatory mediators. Little is known, however, about the response of these cells to mechanical strain with particles, which is often a component of the physical environment of the cell. We therefore studied the production of prostaglandin E(2) (PGE(2)), an important mediator in aseptic loosening and periprosthetic osteolysis in total joint arthroplasties, for human macrophages treated with mechanical stretch alone, titanium particles alone, and mechanical stretch and particles combined. A combination of mechanical stretch and titanium particles resulted in a statistically synergistic elevation of levels of PGE(2) compared with the levels found with either stretch or particles alone. Exposure of human macrophages to mechanical stretch with particles upregulated the expression of cyclooxygenase (COX)-2 mRNA but not COX-1 mRNA, this expression resulting in a 97-fold increase in PGE(2) production compared to the nonstimulated cells. The current study is the first to investigate the effects of mechanical stretch with particles on cultured macrophages and include an investigation of the time course of PGE(2) production and COX-2 mRNA expression. Our results suggest that, while mechanical strain may be one of the primary factors responsible for macrophage activation and periprosthetic osteolysis, mechanical strain with particles load may contribute significantly to the osteolytic potential of macrophages in vitro. The synergistic effect observed between mechanical stretch and particles could accelerate implant loosening and implies that reduction in either cyclic mechanical strain or wear debris load would lead to a reduction of osteolysis.
Takaaki Fujishiro; Tetsuo Nishikawa; Nao Shibanuma; Toshihiro Akisue; Satoshi Takikawa; Tetsuji Yamamoto; Shinichi Yoshiya; Masahiro Kurosaka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  68     ISSN:  1549-3296     ISO Abbreviation:  -     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-05     Completed Date:  2004-10-13     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  531-6     Citation Subset:  IM    
Copyright Information:
Copyright 2004 Wiley Periodicals, Inc.
Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
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MeSH Terms
Cyclooxygenase 2
Dinoprostone / biosynthesis*
Equipment Failure Analysis
Isoenzymes / genetics
Joint Prosthesis / standards*
Macrophages / metabolism*
Membrane Proteins
Osteolysis / etiology
Prostaglandin-Endoperoxide Synthases / genetics
RNA, Messenger / analysis
Stress, Mechanical
Titanium / pharmacology*
Reg. No./Substance:
0/Isoenzymes; 0/Membrane Proteins; 0/RNA, Messenger; 363-24-6/Dinoprostone; 7440-32-6/Titanium; EC 2; EC protein, human; EC Synthases

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