| Effect of cyclic AMP on lipid accumulation and metabolism in human atherosclerotic aortic cells. | |
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MedLine Citation:
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PMID: 2430588 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of dibutyryl cyclic AMP (db cAMP), cholera toxin, and methylisobutylxanthine on the content and metabolism of lipids in smooth muscle cells cultured from normal and atherosclerotic intima of human aorta have been studied. Db cAMP (0.1 mM) decreased the levels of triglycerides and esterified sterols 1.5-3-fold in cells cultured from fatty streaks and atherosclerotic plaques. Cholera toxin (100 micrograms/ml) and methylisobutylxanthine (0.1 mM) stimulated by 2-fold the cyclic AMP level in aortic smooth muscle cells and decreased the content of triglycerides and esterified sterols by 2-3-fold. Prolonged treatment with db cAMP and methylisobutylxanthine resulted in a fall in the intracellular phospholipids and free sterols. Using the labelled precursors, [3H]acetate and [14C]oleate, it was established that the agents increasing the intracellular cyclic AMP level inhibit the formation of sterols and fatty acids, impede the synthesis of phospholipids, triglycerides and esterified sterols, and stimulate their hydrolysis. The data demonstrate that cyclic AMP facilitates the regression of cellular lipoidosis by altering the intracellular lipid metabolism. |
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Authors:
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V V Tertov; A N Orekhov; V N Smirnov |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Atherosclerosis Volume: 62 ISSN: 0021-9150 ISO Abbreviation: Atherosclerosis Publication Date: 1986 Oct |
Date Detail:
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Created Date: 1986-12-15 Completed Date: 1986-12-15 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0242543 Medline TA: Atherosclerosis Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 55-64 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-3-isobutylxanthine
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pharmacology Aorta / drug effects, metabolism*, pathology Arteriosclerosis / metabolism*, pathology Bucladesine / pharmacology* Cells, Cultured Cholera Toxin / pharmacology Cyclic AMP / metabolism* Humans Kinetics Lipid Metabolism* Male Middle Aged Muscle, Smooth, Vascular / drug effects, metabolism, pathology |
| Chemical | |
Reg. No./Substance:
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28822-58-4/1-Methyl-3-isobutylxanthine; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP; 9012-63-9/Cholera Toxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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