Document Detail

Effect of culture media on in vitro development of cloned mouse embryos.
MedLine Citation:
PMID:  11900638     Owner:  NLM     Status:  MEDLINE    
The effect of simple and sequential embryo culture media on the preimplantation development of mouse nuclear transfer (NT) embryos reconstructed with cumulus cell nuclei using a mechanical NT technique was studied. Blastocyst formation rate was evaluated using CZB medium and the sequential media G1/G2 and KSOM/G2. Arrested two- and three-cell NT embryos were Hoechst-stained to check for nuclear abnormalities. Nonmanipulated and sham-manipulated parthenogenetic embryos served as controls for, respectively, the medium and the handling technique. Rates of blastocyst formation for medium and handling control embryos were similar in CZB (58% and 61%), in G1/G2 (94% and 85%), and in KSOM/G2 (88% and 84%). Development of NT embryos was significantly impaired from the two-cell stage onwards, reaching the blastocyst stage at a rate of 5% in CZB, 14% in G1/G2, and 28% in KSOM/G2. Arrested two- and three-cell stage NT embryos showed a high rate of binucleation. These data demonstrate not only that NT embryos are more sensitive to in vitro culture conditions than parthenogenetic control embryos but also that selection of culture media can influence the preimplantation development of NT embryos.
B Heindryckx; A Rybouchkin; J Van Der Elst; M Dhont
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cloning     Volume:  3     ISSN:  1520-4553     ISO Abbreviation:  Cloning     Publication Date:  2001  
Date Detail:
Created Date:  2002-03-19     Completed Date:  2002-05-20     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  100883430     Medline TA:  Cloning     Country:  United States    
Other Details:
Languages:  eng     Pagination:  41-50     Citation Subset:  IM    
Infertility Centre, Ghent University Hospital, Ghent, Belgium.
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MeSH Terms
Blastocyst / physiology
Cell Nucleus / metabolism
Cloning, Organism / methods*
Culture Media / pharmacology*
Mice, Inbred C57BL
Time Factors
Zygote / physiology
Reg. No./Substance:
0/Culture Media

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