Document Detail

Effect of cultivation conditions on spore production from Bacillus amyloliquefaciens B128 and its antagonism to Botrytis elliptica.
MedLine Citation:
PMID:  18179538     Owner:  NLM     Status:  MEDLINE    
AIMS: To maximize spore production by Bacillus amyloliquefaciens B128, and its antagonism to the fungal pathogen Botrytis elliptica B061. METHODS AND RESULTS: In the 5-l stirred-tank bioreactor (STR), with the 0.5 vvm aeration rate, an agitation rate of 200 rev min(-1) significantly enhanced the spore yield compared to the same in 300 rev min(-1) cultivations. In a 20-l airlift bioreactor (ALR) the maximal spore production was further increased with a controlled aeration rate of 2.5 vvm operated in a 24-mesh net-draft tube mode, and no pH control cultivation. This spore yield in the 20-l ALR was five- and eightfold higher; in addition the cultivation period was 19 h shorter, compared to that obtained from shaker flask and in the 5-l STR cultivations respectively. CONCLUSIONS: Although culture conditions are still to be optimized, by using an ALR with net-draft tube, a scaling up from shaker flasks and STR to ALR of spore production by the strain B128 is technically feasible. SIGNIFICANCE AND IMPACT OF THE STUDY: The spore yields obtained using bioreactors were much higher than those previously reported. The freshly produced spore preparations from the B128 strain significantly antagonized the grey mould pathogen B. elliptica.
Y-M Tzeng; Y K Rao; K-J Tsay; W-S Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-20
Journal Detail:
Title:  Journal of applied microbiology     Volume:  104     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-16     Completed Date:  2008-09-02     Revised Date:  2010-03-24    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1275-82     Citation Subset:  IM    
Institute of Biotechnology, Chaoyang University of Technology, Wufeng, Taiwan, Republic of China.
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MeSH Terms
Antibiosis / physiology*
Bacillus / physiology*
Botrytis / physiology*,  ultrastructure
Industrial Microbiology*
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Spores, Bacterial / physiology

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