| Effect of cultivation conditions on spore production from Bacillus amyloliquefaciens B128 and its antagonism to Botrytis elliptica. | |
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MedLine Citation:
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PMID: 18179538 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To maximize spore production by Bacillus amyloliquefaciens B128, and its antagonism to the fungal pathogen Botrytis elliptica B061. METHODS AND RESULTS: In the 5-l stirred-tank bioreactor (STR), with the 0.5 vvm aeration rate, an agitation rate of 200 rev min(-1) significantly enhanced the spore yield compared to the same in 300 rev min(-1) cultivations. In a 20-l airlift bioreactor (ALR) the maximal spore production was further increased with a controlled aeration rate of 2.5 vvm operated in a 24-mesh net-draft tube mode, and no pH control cultivation. This spore yield in the 20-l ALR was five- and eightfold higher; in addition the cultivation period was 19 h shorter, compared to that obtained from shaker flask and in the 5-l STR cultivations respectively. CONCLUSIONS: Although culture conditions are still to be optimized, by using an ALR with net-draft tube, a scaling up from shaker flasks and STR to ALR of spore production by the strain B128 is technically feasible. SIGNIFICANCE AND IMPACT OF THE STUDY: The spore yields obtained using bioreactors were much higher than those previously reported. The freshly produced spore preparations from the B128 strain significantly antagonized the grey mould pathogen B. elliptica. |
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Authors:
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Y-M Tzeng; Y K Rao; K-J Tsay; W-S Wu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-20 |
Journal Detail:
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Title: Journal of applied microbiology Volume: 104 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-16 Completed Date: 2008-09-02 Revised Date: 2010-03-24 |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1275-82 Citation Subset: IM |
Affiliation:
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Institute of Biotechnology, Chaoyang University of Technology, Wufeng, Taiwan, Republic of China. ymtzeng@cyut.edu.tw |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibiosis
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physiology* Bacillus / physiology* Bacteriology Bioreactors Botrytis / physiology*, ultrastructure Industrial Microbiology* Microscopy, Electron, Scanning Microscopy, Electron, Transmission Spores, Bacterial / physiology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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