Document Detail


Effect of cultivation conditions on spore production from Bacillus amyloliquefaciens B128 and its antagonism to Botrytis elliptica.
MedLine Citation:
PMID:  18179538     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To maximize spore production by Bacillus amyloliquefaciens B128, and its antagonism to the fungal pathogen Botrytis elliptica B061. METHODS AND RESULTS: In the 5-l stirred-tank bioreactor (STR), with the 0.5 vvm aeration rate, an agitation rate of 200 rev min(-1) significantly enhanced the spore yield compared to the same in 300 rev min(-1) cultivations. In a 20-l airlift bioreactor (ALR) the maximal spore production was further increased with a controlled aeration rate of 2.5 vvm operated in a 24-mesh net-draft tube mode, and no pH control cultivation. This spore yield in the 20-l ALR was five- and eightfold higher; in addition the cultivation period was 19 h shorter, compared to that obtained from shaker flask and in the 5-l STR cultivations respectively. CONCLUSIONS: Although culture conditions are still to be optimized, by using an ALR with net-draft tube, a scaling up from shaker flasks and STR to ALR of spore production by the strain B128 is technically feasible. SIGNIFICANCE AND IMPACT OF THE STUDY: The spore yields obtained using bioreactors were much higher than those previously reported. The freshly produced spore preparations from the B128 strain significantly antagonized the grey mould pathogen B. elliptica.
Authors:
Y-M Tzeng; Y K Rao; K-J Tsay; W-S Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-20
Journal Detail:
Title:  Journal of applied microbiology     Volume:  104     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-16     Completed Date:  2008-09-02     Revised Date:  2010-03-24    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1275-82     Citation Subset:  IM    
Affiliation:
Institute of Biotechnology, Chaoyang University of Technology, Wufeng, Taiwan, Republic of China. ymtzeng@cyut.edu.tw
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MeSH Terms
Descriptor/Qualifier:
Antibiosis / physiology*
Bacillus / physiology*
Bacteriology
Bioreactors
Botrytis / physiology*,  ultrastructure
Industrial Microbiology*
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Spores, Bacterial / physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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