Document Detail


Effect of cultivating conditions on alpha-galactosidase production by a novel Aspergillus foetidus ZU-G1 strain in solid-state fermentation.
MedLine Citation:
PMID:  17542067     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The work is intended to achieve optimum culture conditions of alpha-galactosidase production by a mutant strain Aspergillus foetidus ZU-G1 in solid-state fermentation (SSF). Certain fermentation parameters involving moisture content, incubation temperature, cultivation period of seed, inoculum volume, initial pH value, layers of pledget, load size of medium and period of cultivation were investigated separately. The optimal cultivating conditions of alpha-galactosidase production in SSF were 60% initial moisture of medium, 28 degrees C incubation temperature, 18 h cultivation period of seed, 10% inoculum volume, 5.0 approximately 6.0 initial pH of medium, 6 layers of pledget and 10 g dry matter loadage. Under the optimized cultivation conditions, the maximum alpha-galactosidase production was 2 037.51 U/g dry matter near the 144th hour of fermentation.
Authors:
Cai-qin Liu; Qi-he Chen; Qian-jun Cheng; Jin-ling Wang; Guo-qing He
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of Zhejiang University. Science. B     Volume:  8     ISSN:  1673-1581     ISO Abbreviation:  J Zhejiang Univ Sci B     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-06-01     Completed Date:  2007-07-17     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  101236535     Medline TA:  J Zhejiang Univ Sci B     Country:  China    
Other Details:
Languages:  eng     Pagination:  371-6     Citation Subset:  IM    
Affiliation:
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China.
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MeSH Terms
Descriptor/Qualifier:
Aspergillus / classification,  enzymology*,  growth & development*
Cell Culture Techniques / methods*
Enzyme Activation
Enzyme Stability
Fermentation
Hydrogen-Ion Concentration
Species Specificity
Temperature
alpha-Galactosidase / biosynthesis*,  chemistry*
Chemical
Reg. No./Substance:
EC 3.2.1.22/alpha-Galactosidase
Comments/Corrections

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