| Effect of cryopreservation on lipids and some physiological features of spermatozoa from rams pastured in highlands and in valleys. | |
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MedLine Citation:
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PMID: 10643642 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of low temperature preservation on the motility and morphology of acrosomes, acrosomal proteolytic activity, phospholipid and fatty acid composition of phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE), and the cholesterol/phospholipid molar ratio in sperm from rams housed in the highlands or in the valleys, were studied. The indices of motility and morphological integrity of sperm from highland rams were much greater compared with those of valley rams. Phosphatidyl choline (PC) of the highland rams was more unsaturated, while PE was more saturated compared with those of valley rams. Cryopreservation of the sperm from highland rams significantly increased the content of choline plasmalogen, accompanied by a slight rise in the levels of lysophosphatidyl choline (LPC) and phosphatidyl inositol (PI) in their sperm. The fatty acid composition altered following cryopreservation. These variations were mainly due to a decrease in the amount of docosahexaenic acid and an increase in the amounts of linoleic and palmitic fatty acids. The results may be indicative of the fact that the alterations in the sperm of the valley rams were more pronounced and they may be attributed to the structural features of the sperm, as well as a reduced concentration of oxygen in the organs and tissues of the highland rams. |
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Authors:
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S I Drokin; T N Vaisberg; E F Kopeika; K D Miteva; G L Pironcheva |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytobios Volume: 100 ISSN: 0011-4529 ISO Abbreviation: Cytobios Publication Date: 1999 |
Date Detail:
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Created Date: 2000-03-16 Completed Date: 2000-03-16 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0207227 Medline TA: Cytobios Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 27-36 Citation Subset: IM |
Affiliation:
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Department of Cryobiology of the Reproductive System, Ukrainian Academy of Sciences, Kharkov, Ukraine. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrosome
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physiology Adaptation, Biological Animals Cryopreservation* Fatty Acids / metabolism Male Phospholipids / metabolism Semen Preservation* Sheep Sperm Motility Spermatozoa* / physiology |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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