Document Detail

Effect of cryopreservation on the immunogenicity of umbilical cord blood cells.
MedLine Citation:
PMID:  14746821     Owner:  NLM     Status:  MEDLINE    
Cryopreservation is the accepted method for long-term storage of cord blood (CB) cells. We evaluated the effects of using different cooling rates (1, 5, 7.5 and 10 degrees C/min) on CB cell allostimulatory and alloproliferative function, antigen expression and clonogenic potential. Significant decreases (P<0.001-0.003) in viable cell recovery observed between fresh CB cells and CB cells cryopreserved at each cooling rate tested. Reductions in clonogenic potential of CB cells cryopreserved at cooling rates of 1, 5, 7.5 and 10 degrees C/min were 44%, 76%, 88% and 93% respectively, compared to fresh controls. FACS analysis indicated no changes in percentages CD34+ cells or lymphocytes. Two sets of mixed lymphocyte reactions were carried out for each CB sample. It was observed that allostimulatory and alloproliferative function varied following cryopreservation at different cooling rates (1 and 5 degrees C/min). Interestingly, there was a significant decrease (P<0.001-0.04) in the alloproliferative function of six of the seven CB samples following cryopreservation using a cooling rate of 5 degrees C/min. Cooling rates between 1 and 5 degrees C/min may provide immunomodulation of CB with maintenance of haematopoietic progenitor cells function.
Natkunam Ketheesan; Cass Whiteman; Agnieszka B Malczewski; Robert G Hirst; Justin T La Brooy
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis     Volume:  30     ISSN:  1473-0502     ISO Abbreviation:  Transfus. Apher. Sci.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-28     Completed Date:  2004-04-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101095653     Medline TA:  Transfus Apher Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  47-54     Citation Subset:  T    
Microbiology and Immunology, James Cook University, 4811 Queensland, Australia.
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MeSH Terms
Antigens, CD34 / biosynthesis
Blood Preservation*
Cell Division
Cell Separation / methods
Cell Survival
Cells, Cultured
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Endothelial Cells / cytology*
Fetal Blood / cytology*
Flow Cytometry
Hematopoietic Stem Cells / cytology
Histocompatibility Testing
Leukocytes, Mononuclear / cytology
Lymphocytes / cytology,  metabolism
Time Factors
Umbilical Veins / cytology*
Reg. No./Substance:
0/Antigens, CD34; 0/Cryoprotective Agents

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