| Effect of covalently bound fatty acids and associated lipids on the viscosity of gastric mucus glycoprotein in cystic fibrosis. | |
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MedLine Citation:
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PMID: 2427379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The contribution of associated and covalently bound lipids to the viscosity of gastric mucus glycoprotein in healthy and cystic fibrosis (CF) individuals was investigated. While both preparations exhibited similar contents of protein and carbohydrate, the CF glycoprotein contained 1.3 times more associated lipids and 6 times more covalently bound fatty acids. The viscosity of CF mucus glycoprotein was about 1.8 times higher than that of normal glycoprotein. Extraction of associated lipids lead to 3-fold drop in the viscosity of CF glycoprotein and 5-fold drop in the case of normal glycoprotein. Removal of covalently bound fatty acids caused further 1.6-fold reduction in the viscosity of normal mucus glycoprotein and 6-fold in CF glycoprotein. The viscosity of the delipidated and deacylated CF mucus glycoprotein was only about 10% higher than that of the similarly treated normal glycoprotein. The results suggest that the elevated level of covalently bound and associated lipids is responsible for the increased viscosity of CF mucin. |
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Authors:
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B L Slomiany; V L Murty; S R Carter; A Slomiany |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Digestion Volume: 34 ISSN: 0012-2823 ISO Abbreviation: Digestion Publication Date: 1986 |
Date Detail:
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Created Date: 1986-10-08 Completed Date: 1986-10-08 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0150472 Medline TA: Digestion Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 275-80 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Carbohydrates / analysis Child Child, Preschool Cystic Fibrosis / metabolism* Fatty Acids / analysis* Female Gastric Mucins / analysis Gastric Mucosa / secretion* Glycoproteins / analysis* Humans Lipids / analysis* Male Mucus / analysis* Viscosity |
| Grant Support | |
ID/Acronym/Agency:
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AM21684-08/AM/NIADDK NIH HHS; HL32553-01/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Fatty Acids; 0/Gastric Mucins; 0/Glycoproteins; 0/Lipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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