Document Detail


Effect of covalently bound fatty acids and associated lipids on the viscosity of gastric mucus glycoprotein in cystic fibrosis.
MedLine Citation:
PMID:  2427379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The contribution of associated and covalently bound lipids to the viscosity of gastric mucus glycoprotein in healthy and cystic fibrosis (CF) individuals was investigated. While both preparations exhibited similar contents of protein and carbohydrate, the CF glycoprotein contained 1.3 times more associated lipids and 6 times more covalently bound fatty acids. The viscosity of CF mucus glycoprotein was about 1.8 times higher than that of normal glycoprotein. Extraction of associated lipids lead to 3-fold drop in the viscosity of CF glycoprotein and 5-fold drop in the case of normal glycoprotein. Removal of covalently bound fatty acids caused further 1.6-fold reduction in the viscosity of normal mucus glycoprotein and 6-fold in CF glycoprotein. The viscosity of the delipidated and deacylated CF mucus glycoprotein was only about 10% higher than that of the similarly treated normal glycoprotein. The results suggest that the elevated level of covalently bound and associated lipids is responsible for the increased viscosity of CF mucin.
Authors:
B L Slomiany; V L Murty; S R Carter; A Slomiany
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Digestion     Volume:  34     ISSN:  0012-2823     ISO Abbreviation:  Digestion     Publication Date:  1986  
Date Detail:
Created Date:  1986-10-08     Completed Date:  1986-10-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0150472     Medline TA:  Digestion     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  275-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Carbohydrates / analysis
Child
Child, Preschool
Cystic Fibrosis / metabolism*
Fatty Acids / analysis*
Female
Gastric Mucins / analysis
Gastric Mucosa / secretion*
Glycoproteins / analysis*
Humans
Lipids / analysis*
Male
Mucus / analysis*
Viscosity
Grant Support
ID/Acronym/Agency:
AM21684-08/AM/NIADDK NIH HHS; HL32553-01/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Fatty Acids; 0/Gastric Mucins; 0/Glycoproteins; 0/Lipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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