Document Detail


Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata.
MedLine Citation:
PMID:  19768555     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence of copper in water environment may have detrimental effects on aquatic organisms, including algae, where different enzymatic systems can be affected. Algae acid phosphatase plays important roles in metabolic processes such as decomposition of organic phosphate, autophagic digestive process, recycling cellular materials and zygote formation during reproduction. This work describes an in vitro activation effect of copper on the acid phosphatase of the green algae Pseudokirchneriella subcapitata (formely Selenastrum capricornutum) under preincubation condition. Apparent Michaelis constant values of 1.21 and 0.37 mM, and activation energy values of 26.8 and 13.6 kJ mol(-1) were determined in the absence and in the presence of 0.2 mM Cu(2+), respectively. The dissociation constant value for Cu(2+) binding to the enzyme was determined to be 22.04 microM. The decrease of the apparent Michaelis constant (Km) and activation energy values in the presence of Cu(2+) correlates well with its activating effect on the acid phosphatase activity. This propriety could be used as a sensitive bioindicator for copper in environmental samples.
Authors:
Claudio Mart?n Jonsson; Hiroshi Aoyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-19
Journal Detail:
Title:  Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine     Volume:  23     ISSN:  1572-8773     ISO Abbreviation:  Biometals     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-04-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208478     Medline TA:  Biometals     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  93-8     Citation Subset:  IM    
Affiliation:
Embrapa Meio Ambiente, Jaguari?na, S?o Paulo, Brazil. jonsson@cnpma.embrapa.br
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / antagonists & inhibitors*,  chemistry,  metabolism
Algae, Green / enzymology*,  growth & development
Copper Sulfate / chemistry,  pharmacology*
Enzyme Activation / drug effects
Enzyme Stability / drug effects
Kinetics
Structure-Activity Relationship
Chemical
Reg. No./Substance:
7758-98-7/Copper Sulfate; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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