Document Detail

Effect of consumed carbon to nitrogen ratio of mycelial morphology and arachidonic acid production in cultures of Mortierella alpina.
MedLine Citation:
PMID:  16233009     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The influence of the consumed carbon to nitrogen (C/N) ratio on arachidonic acid (AA) production and mycelial morphology was investigated in cultures of Mortierella alpina using shake flasks and a fermentor. The consumed C/N ratio was varied from 5 to 32 under the condition that the total initial amount of carbon and nitrogen sources was 50 g/l. Cellular yield increased markedly at C/N ratios below 7; carbon utilization was switched from cellular growth to lipid biosynthesis in the C/N ratio range of 7-15; lipid biosynthesis was most active when the C/N ratio was in the range of 15-32. However, for C/N ratios higher than 15, the mycelial concentration decreased due to nitrogen limitation but the lipid yield still increased. In the presence of excess nitrogen, the biomass concentration depended on the amount of the nitrogen source, but the AA yield was inversely related to this. On the other hand, in the presence of excess carbon, the fatty acid concentration increased with carbon source concentration but the AA concentration remained constant. From the viewpoint of AA production, the optimum C/N ratio was in the range of 15 to 20 with a balance between the amounts of carbon and nitrogen sources. When an enriched medium was used at a fixed C/N ratio of 20, the cellular and AA concentrations were shown to be proportional to the total concentrations of carbon and nitrogen sources in both flasks and the fermentor. The whole pellet size and width of pellet annular regions did not change with increasing C/N ratio for C/N ratios below 20 in the flask cultures. However, when the C/N ratio was higher than 20, these sizes increased in proportion to the C/N ratio.
Y Koike; H J Cai; K Higashiyama; S Fujikawa; E Y Park
Related Documents :
14273639 - Pyruvate metabolism, carbon dioxide assimilation, and nitrogen fixation by an achromoba...
192859 - Evaluation of formamidine sulfinic acid and other reducing agents for use in the prepar...
10683809 - Effect of a new amino acid solution on nutritional status and nitrogen metabolism in ra...
16216399 - Export of nutrients from golf courses on the precambrian shield.
7305899 - The mechanism of ammonia production and the effect of mechanical work load on proteolys...
16902329 - Amino acid requirements of infants and children.
11959859 - Functional characterization of two novel mammalian electrogenic proton-dependent amino ...
6325649 - Ultrastructural localization of acid phosphatase activity in plasma cells containing ru...
290749 - Structural specificity of the carrier for l-tryptophan in rat cerebral cortex slices.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  91     ISSN:  1389-1723     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2001  
Date Detail:
Created Date:  2005-10-19     Completed Date:  2005-11-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  382-9     Citation Subset:  -    
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A second pectin lyase gene (pel2) from Aspergillus oryzae KBN616: its sequence analysis and overexpr...
Next Document:  Characterization of bacteriocin N15 produced by Enterococcus faecium N15 and cloning of the related ...