| Effect of constitutively active ras overexpression on cell growth in recombinant chinese hamster ovary cells. | |
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MedLine Citation:
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PMID: 21438179 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Constitutively active Ras (CA-Ras) is known to enhance cell growth through the induction of various signaling cascades including the phosphoinositide 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK)/ERK signaling pathways, although the cellular response is highly dependent on the cell type. To evaluate the effect of CA-Ras overexpression on cell growth in recombinant Chinese hamster ovary (rCHO) cells, an erythropoietin (EPO)-producing rCHO cell line with regulated CA-Ras overexpression (EPO-off-CA-Ras) was established using the Tet-off system. The CA-Ras expression level in EPO-off-CA-Ras cells was tightly regulated by doxycycline addition. Although CA-Ras overexpression slightly increased the viable cell concentration during the late exponential phase, it did not increase the maximum viable cell concentration or specific growth rate to a significant degree. Unexpectedly, CA-Ras overexpression in rCHO cells led only to the enhancement in the activation of the MAPK/ERK signaling pathway and not the PI3K/Akt signaling pathway. Taken together, CA-Ras overexpression in rCHO cells did not significantly affect cell growth; it also had no critical impact on viable cell concentration or EPO production, possibly due to a failure to activate the PI3K/Akt signaling pathway. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011. |
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Authors:
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Yeon-Gu Kim; Young Kue Han; Jee Yon Kim; Eun Gyo Lee; Hong Weon Lee; Gyun Min Lee |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-25 |
Journal Detail:
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Title: Biotechnology progress Volume: - ISSN: 1520-6033 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-3-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 American Institute of Chemical Engineers (AIChE). |
Affiliation:
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Dept. of Biological Sciences, Graduate School of Nanoscience and Technology (WCU), KAIST, Daejon 305-701, Korea; Biotechnology Process Engineering Center, KRIBB, Daejeon 305-806, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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