| Effect of a conjugated quercetin metabolite, quercetin 3-glucuronide, on lipid hydroperoxide-dependent formation of reactive oxygen species in differentiated PC-12 cells. | |
| | |
MedLine Citation:
|
PMID: 17015249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To assess the efficacy of conjugated quercetin metabolites as attenuators for oxidative stress in the central nervous system, we measured the 13-hydroperoxyoctadecadienoic acid (13-HPODE)-dependent formation of reactive oxygen species (ROS) in pheochromocytoma PC-12 cells in the presence of quercetin 3-O-beta-glucuronide (Q3GA) and related compounds. A 2',7'-dichlorofluorescin (DCFH) assay showed that Q3GA significantly suppressed the formation of ROS, when it was coincubated with 13-HPODE (coincubation system). However, it was less effective than quercetin aglycon in the concentration range from 0.5 to 10 microM. In an experiment in which the cells were incubated with the test compounds for 24 h before being exposed to 13-HPODE, Q3GA was also effective in suppressing the formation of ROS in spite that little Q3GA was taken up into the cells. These results suggest that antioxidative metabolites of quercetin are capable of protecting nerve cells from attack of lipid hydroperoxides. |
| | |
Authors:
|
Mutsuko Shirai; Yoshichika Kawai; Rintaro Yamanishi; Takashi Kinoshita; Hiroshi Chuman; Junji Terao |
Related Documents
:
|
15638999 - Inhibition of nox-4 activity by plumbagin, a plant-derived bioactive naphthoquinone. 10935319 - Superoxide anions mediate proliferative response in cardiac fibroblasts. 6094369 - In vitro determination of phagocyte activity by luminol- and lucigenin-amplified chemil... 3801479 - Oxygen radicals alter the cell membrane potential in a renal cell line (llc-pk1) with d... 20581459 - Exposure to acute hypoxia induces a transient dna damage response which includes chk1 a... 7796889 - Cell cycle-related changes in f-actin distribution are correlated with glycolytic activ... 21519159 - Establishment and characterization of canine rhabdomyosarcoma cell line, cms-c. 1353079 - Monoclonal antibodies against the extracellular domain of the erbb-2 receptor function ... 6776019 - Effect of some benzthiazol derivatives on euglena gracilis. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Free radical research Volume: 40 ISSN: 1071-5762 ISO Abbreviation: Free Radic. Res. Publication Date: 2006 Oct |
Date Detail:
|
Created Date: 2006-10-03 Completed Date: 2006-11-08 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
|
Languages: eng Pagination: 1047-53 Citation Subset: IM |
Affiliation:
|
Department of Food Science, Institute for Health Biosciences, The University of Tokushima Graduate School, Kuramoto-cho 3, Tokushima 770-8503, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Differentiation Free Radical Scavengers / metabolism, pharmacology* Linoleic Acids / toxicity Lipid Peroxidation / drug effects* Lipid Peroxides / toxicity Neurons / drug effects*, metabolism Neuroprotective Agents / metabolism, pharmacology* Oxidative Stress / drug effects PC12 Cells Quercetin / analogs & derivatives*, metabolism, pharmacology Rats Reactive Oxygen Species / analysis, antagonists & inhibitors*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Free Radical Scavengers; 0/Linoleic Acids; 0/Lipid Peroxides; 0/Neuroprotective Agents; 0/Reactive Oxygen Species; 0/quercetin 3-O-glucuronide; 117-39-5/Quercetin; 23017-93-8/13-hydroperoxy-9,11-octadecadienoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The relative contribution of the small and large intestine to the absorption and metabolism of rutin...
Next Document: Endothelial function and cardiovascular disease: effects of quercetin and wine polyphenols.