| Effect of conditioned medium factors on productivity and cell physiology in Trichoplusia ni insect cell cultures. | |
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MedLine Citation:
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PMID: 16739946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The influence of conditioned medium (CM) on cell physiology and recombinant protein production in Trichoplusia ni insect cells (T. ni, BTI-Tn-5B1-4) has been investigated. Cell cycle analysis showed that a high proportion of the cell population (80-90%) was in G1 during the whole culture, indicating that the S and G2/M phases are short relative to the G1 phase. Directly after inoculation, a rapid decrease of the S-phase population occurred, which could be observed as a lag-phase. The following increase in the number of cells in S occurred after 7 h of culture for cells in fresh medium, whereas for cells with the addition of CM it occurred at an earlier time point (5 h) and these cells had therefore a shorter lag-phase. The initial changes in the S-phase population were also affected by the inoculum cell density, as higher seeding cell densities resulted in a more rapid increase in the S-phase population after inoculation. These changes in cell cycle distribution were reflected in the cell size, and the CM-cells were smaller than the cells in fresh medium. Recombinant protein production in T. ni cells was improved by the addition of CM. The specific productivity was increased by 30% compared to cells in fresh medium. This beneficial effect was seen between 20 and 72 h of culture. In contrast, the highest specific productivity was obtained already at 7 h for the cells in fresh medium and then decreased rapidly. The total product concentration was around 30% higher in the culture with CM compared to the culture in fresh medium, and the maximum product concentration was obtained on day 2 compared to day 3 for the cells in fresh medium. Our results indicate that the positive effect on productivity by CM is related to its growth-promoting effect, suggesting that the proliferation potential of the culture determines the productivity. |
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Authors:
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Karin Calles; Ulrika Eriksson; Lena Häggström |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology progress Volume: 22 ISSN: 8756-7938 ISO Abbreviation: Biotechnol. Prog. Publication Date: 2006 May-Jun |
Date Detail:
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Created Date: 2006-06-02 Completed Date: 2006-08-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: United States |
Other Details:
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Languages: eng Pagination: 653-9 Citation Subset: IM |
Affiliation:
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Karo Bio AB, Huddinge, SE-141 57 Huddinge, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Culture Techniques / methods Cell Cycle / drug effects Cell Line Cell Proliferation / drug effects Cells, Cultured Culture Media, Conditioned / pharmacology* Insect Proteins / biosynthesis*, drug effects Moths / cytology, metabolism* Recombinant Proteins / biosynthesis, drug effects Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Insect Proteins; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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