Document Detail

Effect of concentration and hyaluronidase on restriction of hetastarch flux through lung interstitial segments.
MedLine Citation:
PMID:  14609528     Owner:  NLM     Status:  MEDLINE    
The transport properties of lung interstitium were studied by measuring the flow of hetastarch solution (2 and 6%) through 1-cm perivascular interstitial segments of rabbit lungs. Hetastarch (10(4)-10(7) Da) solution has a colloid osmotic pressure similar to that of albumin solution. Driving pressure was 5 cm H(2)O and mean interstitial pressure was 0 cm H(2)O. The flows of 2 and 6% hetastarch solutions were measured before (Q(1)) and after (Q(2)) the addition of 0.02% hyaluronidase. Hetastarch molecular distributions in effluent samples were measured by high-performance size-exclusion chromatography (HPSEC) to determine sieving ratio (C(out)/C(in), downstream-to-upstream concentration ratio). Hyaluronidase significantly (P < 0.0004) increased flow sixfold, but the increase in flow (Q(2)/Q(1)) was reduced through the interstitium around smaller vessels. A similar behavior was observed with the flow of albumin solution without and with hyaluronidase. C(out)/C(in) decreased monotonically with molecular weight, was greater with 6% than with 2% (low colloid osmotic pressure) hetastarch, and increased with hyaluronidase. Modeling the transport through uniform pores, equivalent pore radius was 10 and 15 nm with 2 and 6% hetastarch, respectively, and doubled with hyaluronidase. In conclusion, interstitial pores expand in response to an increase in colloid osmotic pressure both before and after tissue degradation by hyaluronidase.
N E Aronson; P K Houtz; S Villarruel; R L Conhaim; K E Watson; S J Lai-Fook
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Microvascular research     Volume:  66     ISSN:  0026-2862     ISO Abbreviation:  Microvasc. Res.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-11     Completed Date:  2004-07-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0165035     Medline TA:  Microvasc Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  218-26     Citation Subset:  IM    
Center for Biomedical Engineering, University of Kentucky, Lexington, KY 40506-0070, USA.
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MeSH Terms
Chromatography, High Pressure Liquid
Hetastarch / pharmacology*
Hyaluronoglucosaminidase / metabolism,  pharmacology*
Lung / drug effects,  pathology*
Models, Theoretical
Osmotic Pressure
Plasma Substitutes / pharmacology
Time Factors
Water / chemistry
Grant Support
Reg. No./Substance:
0/Plasma Substitutes; 7732-18-5/Water; 9005-27-0/Hetastarch; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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