Document Detail

Effect of citrus lemon oil on growth and adherence of Streptococcus mutans.
MedLine Citation:
PMID:  23381618     Owner:  NLM     Status:  MEDLINE    
In order to exploit novel anticaries agents, we investigated the effects of citrus lemon oil (CLO), a type of natural product, on growth and adherence of the primary oral cariogenic bacteria Streptococcus mutans (S. mutans). The growth inhibitory effect was explored with a micro-dilution assay. Adherence was analyzed by colony counts on the respective surfaces and the adherence inhibition rate (AIR). Real time-PCR was used to investigate the effects of CLO on transcription of glucosyltransferase (Gtf) encoding genes, gtfB, C and D. Neson-Somogyi method was used to measure the effects of CLO on Gtf activity. The minimum inhibitory concentration of CLO against S. mutans was 4.5 mg/ml. The CLO effectively reduced the adherence of S. mutans on glass surface (the AIR were from 98.3 to 100 %, P > 0.05) and saliva-coated enamel surface (the AIR were from 54.8 to 79.2 %, P < 0.05). CLO effectively reduced the activity of Gtf and the transcription of gtfs in a dose dependent manner (P < 0.05). In conclusion, CLO can effectively inhibit the growth and the adherence to glass and saliva-coated enamel surfaces of S. mutans. It can also inhibit the transcription of gtfs, as well as the Gtf enzyme activity.
Ying Liu; Xiangyu Zhang; Yuzhi Wang; Feifei Chen; Zhifen Yu; Li Wang; Shuanglu Chen; Maoding Guo
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Publication Detail:
Type:  Journal Article     Date:  2013-02-05
Journal Detail:
Title:  World journal of microbiology & biotechnology     Volume:  29     ISSN:  1573-0972     ISO Abbreviation:  World J. Microbiol. Biotechnol.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-17     Completed Date:  2013-12-23     Revised Date:  2014-04-22    
Medline Journal Info:
Nlm Unique ID:  9012472     Medline TA:  World J Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1161-7     Citation Subset:  IM    
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MeSH Terms
Anti-Bacterial Agents / isolation & purification,  pharmacology*
Bacterial Adhesion / drug effects*
Citrus / chemistry*
Colony Count, Microbial
Gene Expression / drug effects
Gene Expression Profiling
Microbial Sensitivity Tests
Plant Oils / isolation & purification,  pharmacology*
Real-Time Polymerase Chain Reaction
Streptococcus mutans / drug effects*,  growth & development,  physiology
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Plant Oils; 0/lemon oil
Erratum In:
World J Microbiol Biotechnol. 2014 Apr;30(4):1435

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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