Document Detail

Effect of cigarette smoke extract on neonatal porcine vascular smooth muscle cells.
MedLine Citation:
PMID:  11162777     Owner:  NLM     Status:  MEDLINE    
RATIONALE: Cigarette smoke exposure in the perinatal period increases the risk of various prenatal and postnatal complications, including sudden infant death syndrome (SIDS) and persistent pulmonary hypertension of the newborn (PPHN). We investigated the cellular effects of cigarette smoke extract (CSE) in the developing vasculature. METHODS: Vascular smooth muscle cells (VSMC) were isolated from neonatal porcine carotid arteries, splenic arteries, and main and resistance pulmonary arteries. Effects of CSE on VSMC proliferation, viability, apoptosis, and media nitrates and nitrites were evaluated. The effects of known constituents of CSE (nicotine, benzopyrene, acrolein, acetaldehyde), aged CSE, CSE with added hemoglobin, devolatilized CSE, CSE with added dithiothreitol (DTT), and reduced glutathione (GSH) on cell proliferation and viability were assessed. RESULTS: CSE caused a dose- and time-dependent decrease in neonatal VSMC numbers isolated from all four sites, mainly as a result of increased cell necrosis and not apoptosis. Nitrates and nitrites were below the threshold of detection. Nicotine and benzopyrene did not affect cell counts, while acrolein and acetaldehyde decreased cell counts in a dose-dependent manner. Addition of hemoglobin, devolatilization, and the addition of DTT or GSH slightly decreased CSE inhibition. CONCLUSIONS: CSE causes necrosis of neonatal VSMC, and this toxicity is mediated mainly by volatile components such as acrolein and acetaldehyde, possibly in association with nitric oxide and carbon monoxide.
N Ambalavanan; W F Carlo; A Bulger; J Shi; J B Philips
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  170     ISSN:  0041-008X     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-15     Revised Date:  2010-03-24    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  130-6     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Pediatrics, Division of Neonatology, University of Alabama at Birmingham, 525 New Hillman Bldg., Birmingham, AL 35233-7335, USA.
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MeSH Terms
Acetaldehyde / toxicity
Acrolein / toxicity
Apoptosis / drug effects
Cell Division / drug effects
Cell Survival / drug effects
Cells, Cultured
Culture Media
Muscle, Smooth, Vascular / cytology,  drug effects*
Nicotine / toxicity
Nitrates / metabolism
Nitrites / metabolism
Plants, Toxic*
Smoke / adverse effects*
Reg. No./Substance:
0/Culture Media; 0/Nitrates; 0/Nitrites; 0/Smoke; 107-02-8/Acrolein; 54-11-5/Nicotine; 75-07-0/Acetaldehyde

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